Dual-mode optical microscope based on single-pixel imaging
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Other documents of the author: Rodríguez Jiménez, Ángel David; Clemente Pesudo, Pedro Javier; Tajahuerce, Enrique; Lancis, Jesús
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comunitat-uji-handle3:10234/43643
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Title
Dual-mode optical microscope based on single-pixel imagingAuthor (s)
Date
2016-03xmlui.dri2xhtml.METS-1.0.item-edition
Pre-print, versió de l'autorPublisher
ElsevierISSN
0143-8166Bibliographic citation
RODRÍGUEZ, A. D., et al. Dual-mode optical microscope based on single-pixel imaging. Optics and Lasers in Engineering, 2016, 82: 87-94.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0143816616000348Version
info:eu-repo/semantics/submittedVersionSubject
Abstract
We demonstrate an inverted microscope that can image specimens in both reflection and transmission modes simultaneously with a single light source. The microscope utilizes a digital micromirror device (DMD) for patterned ... [+]
We demonstrate an inverted microscope that can image specimens in both reflection and transmission modes simultaneously with a single light source. The microscope utilizes a digital micromirror device (DMD) for patterned illumination altogether with two single-pixel photosensors for efficient light detection. The system, a scan-less device with no moving parts, works by sequential projection of a set of binary intensity patterns onto the sample that are codified onto a modified commercial DMD. Data to be displayed are geometrically transformed before written into a memory cell to cancel optical artifacts coming from the diamond-like shaped structure of the micromirror array. The 24-bit color depth of the display is fully exploited to increase the frame rate by a factor of 24, which makes the technique practicable for real samples. Our commercial DMD-based LED-illumination is cost effective and can be easily coupled as an add-on module for already existing inverted microscopes. The reflection and transmission information provided by our dual microscope complement each other and can be useful for imaging non-uniform samples and to prevent self-shadowing effects. [-]
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Optics and Lasers in Engineering, Volume 82, July 2016, Pages 87–94Rights
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