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Use of micellar liquid chromatography to analyze oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey and validation according to the 2002/657/EC decision
dc.contributor.author | Tayeb Cherif, K. | |
dc.contributor.author | Vives-Peris, Vicente | |
dc.contributor.author | Carda-Broch, Samuel | |
dc.contributor.author | Esteve-Romero, Josep | |
dc.date.accessioned | 2016-04-21T18:12:22Z | |
dc.date.available | 2016-04-21T18:12:22Z | |
dc.date.issued | 2016-07 | |
dc.identifier.issn | 0308-8146 | |
dc.identifier.uri | http://hdl.handle.net/10234/158915 | |
dc.description.abstract | A micellar liquid chromatographic method was developed for the analysis of oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey. These quinolines are unethically used in beekeeping, and a zero-tolerance policy to antibiotic residues in honey has been stated by the European Union. The sample pretreatment was a 1:1 dilution with a 0.05 M SDS at pH 3 solution, filtration and direct injection, thus avoiding extraction steps. The quinolones were eluted without interferences using mobile phase of 0.05 M SDS/12.5% 1-propanol/0.5% triethylamine at pH 3, running at 1 mL/min under isocratic room through a C18 column. The analytes were detected by fluorescence. The method was successfully validated according to the requirements of the European Union Decision 2002/657/EC in terms of: specificity, linearity (r2 > 0.995), limit of detection and decision limit (0.008–0.070 mg/kg), lower limit of quantification (0.02–0.2 mg/kg), detection capability (0.010–0.10 mg/kg), recovery (82.1–110.0%), precision (<9.4%), matrix effects, robustness (<10.4%), and stability. The procedure was applied to several commercial honey supplied by a local supermarket, and the studied antibiotics were not detected. Therefore, the method was rapid, simple, safe, eco friendly, reliable and useful for the routine analysis of honey samples. | ca_CA |
dc.description.sponsorShip | This work was supported by Project P1.1B2012-36 del Pla de Promoció de la Investigació de la Universitat Jaume I. | |
dc.format.extent | 8 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Food Chemistry, 2016, vol. 202, núm. 1 | ca_CA |
dc.rights | Copyright © 2016 Elsevier Ltd. All rights reserved. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Direct injection | ca_CA |
dc.subject | Honey | ca_CA |
dc.subject | Micellar | ca_CA |
dc.subject | Quinolone | ca_CA |
dc.subject | Validation | ca_CA |
dc.title | Use of micellar liquid chromatography to analyze oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey and validation according to the 2002/657/EC decision | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1016/j.foodchem.2016.02.007 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0308814616301534 | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
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