Use of micellar liquid chromatography to analyze oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey and validation according to the 2002/657/EC decision
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Altres documents de l'autoria: Tayeb Cherif, K.; Vives-Peris, Vicente; Carda-Broch, Samuel; Esteve-Romero, Josep
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Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
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http://dx.doi.org/10.1016/j.foodchem.2016.02.007 |
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Títol
Use of micellar liquid chromatography to analyze oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey and validation according to the 2002/657/EC decisionData de publicació
2016-07Editor
ElsevierISSN
0308-8146Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://www.sciencedirect.com/science/article/pii/S0308814616301534Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
A micellar liquid chromatographic method was developed for the analysis of oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey. These quinolines are unethically used in beekeeping, and a zero-tolerance ... [+]
A micellar liquid chromatographic method was developed for the analysis of oxolinic acid, flumequine, marbofloxacin and enrofloxacin in honey. These quinolines are unethically used in beekeeping, and a zero-tolerance policy to antibiotic residues in honey has been stated by the European Union. The sample pretreatment was a 1:1 dilution with a 0.05 M SDS at pH 3 solution, filtration and direct injection, thus avoiding extraction steps. The quinolones were eluted without interferences using mobile phase of 0.05 M SDS/12.5% 1-propanol/0.5% triethylamine at pH 3, running at 1 mL/min under isocratic room through a C18 column. The analytes were detected by fluorescence. The method was successfully validated according to the requirements of the European Union Decision 2002/657/EC in terms of: specificity, linearity (r2 > 0.995), limit of detection and decision limit (0.008–0.070 mg/kg), lower limit of quantification (0.02–0.2 mg/kg), detection capability (0.010–0.10 mg/kg), recovery (82.1–110.0%), precision (<9.4%), matrix effects, robustness (<10.4%), and stability. The procedure was applied to several commercial honey supplied by a local supermarket, and the studied antibiotics were not detected. Therefore, the method was rapid, simple, safe, eco friendly, reliable and useful for the routine analysis of honey samples. [-]
Publicat a
Food Chemistry, 2016, vol. 202, núm. 1Drets d'accés
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