dc.contributor.author | Martí, Raúl | |
dc.contributor.author | Valcárcel, Mercedes | |
dc.contributor.author | Herrero Martínez, José Manuel | |
dc.contributor.author | Cebolla-Cornejo, Jaime | |
dc.contributor.author | Roselló, Salvador | |
dc.date.accessioned | 2016-04-20T13:59:24Z | |
dc.date.available | 2016-04-20T13:59:24Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | MARTÍ, Raúl, et al. Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column. Food chemistry, 2015, vol. 169, p. 169-179. | ca_CA |
dc.identifier.issn | 0308-8146 | |
dc.identifier.uri | http://hdl.handle.net/10234/158892 | |
dc.description.abstract | A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76–108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD < 2.9%) and retention time (RSD < 0.2%) both for standards and real samples. Detection limits ranged between 3 and 44 μg kg−1 for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs. | ca_CA |
dc.format.extent | 33 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Food chemistry, 2015, vol. 169, p. 169-179 | ca_CA |
dc.rights | Copyright © 2016 Elsevier B.V. or its licensors or contributors. ScienceDirect® is a registered trademark of Elsevier B.V. | ca_CA |
dc.rights | Attribution-NonCommercial-NoDerivs 4.0 Spain | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Functional quality | ca_CA |
dc.subject | HPLC-DAD | ca_CA |
dc.subject | Food | ca_CA |
dc.subject | Ultrasound-assisted extraction | ca_CA |
dc.subject | Response surface methodology | ca_CA |
dc.title | Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1016/j.foodchem.2014.07.151 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0308814614011996 | ca_CA |