Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column
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Other documents of the author: Martí, Raúl; Valcárcel, Mercedes; Herrero Martínez, José Manuel; Cebolla-Cornejo, Jaime; Roselló, Salvador
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comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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Title
Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core columnAuthor (s)
Date
2015Publisher
ElsevierISSN
0308-8146Bibliographic citation
MARTÍ, Raúl, et al. Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column. Food chemistry, 2015, vol. 169, p. 169-179.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0308814614011996Subject
Abstract
A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. ... [+]
A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76–108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD < 2.9%) and retention time (RSD < 0.2%) both for standards and real samples. Detection limits ranged between 3 and 44 μg kg−1 for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs. [-]
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Food chemistry, 2015, vol. 169, p. 169-179Rights
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info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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