Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column
Visualitza/
Impacte
Scholar |
Altres documents de l'autoria: Martí, Raúl; Valcárcel, Mercedes; Herrero Martínez, José Manuel; Cebolla-Cornejo, Jaime; Roselló, Salvador
Metadades
Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
comunitat-uji-handle4:
INVESTIGACIONMetadades
Títol
Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core columnAutoria
Data de publicació
2015Editor
ElsevierISSN
0308-8146Cita bibliogràfica
MARTÍ, Raúl, et al. Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column. Food chemistry, 2015, vol. 169, p. 169-179.Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://www.sciencedirect.com/science/article/pii/S0308814614011996Paraules clau / Matèries
Resum
A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. ... [+]
A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76–108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD < 2.9%) and retention time (RSD < 0.2%) both for standards and real samples. Detection limits ranged between 3 and 44 μg kg−1 for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs. [-]
Publicat a
Food chemistry, 2015, vol. 169, p. 169-179Drets d'accés
Copyright © 2016 Elsevier B.V. or its licensors or contributors. ScienceDirect® is a registered trademark of Elsevier B.V.
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Apareix a les col.leccions
- CAMN_Articles [566]
Els següents fitxers sobre la llicència estan associats a aquest element: