Analysis of the Molecular Dialogue Between Gray Mold (Botrytis cinerea) and Grapevine (Vitis vinifera) Reveals a Clear Shift in Defense Mechanisms During Berry Ripening
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Other documents of the author: Kelloniemi, Jani; Trouvelot, Sophie; Heloir, Marie-Claire; Simon, Adeline; Dalmais, Berengere; Frettinger, Patrick; Cimerman, Agnès; Fermaud, Marc; Roudet, Jean; Baulande, Sylvain; Bruel, Christophe; Choquer, Mathias; Couvelard, Lnhdavanh; Duthieuw, Mathilde; Ferrarini, Alberto; Flors, Victor; Le Pecheur, Pascal; Loise, Elise; Morgant, Guillaume; Poussereau, Nathalie; Pradier, Jean-Marc; Rascle, Christine; Trda, Lucie; Poinssot, Benoit; Viaud, Muriel
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comunitat-uji-handle3:10234/6999
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10.1094/MPMI-02-15-0039-R |
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Title
Analysis of the Molecular Dialogue Between Gray Mold (Botrytis cinerea) and Grapevine (Vitis vinifera) Reveals a Clear Shift in Defense Mechanisms During Berry RipeningAuthor (s)
Date
2015Publisher
Copyright © 2015 The American Phytopathological SocietyISSN
1167-1180Bibliographic citation
KELLONIEMI, Jani, et al. Analysis of the Molecular Dialogue Between Gray Mold (Botrytis cinerea) and Grapevine (Vitis vinifera) Reveals a Clear Shift in Defense Mechanisms During Berry Ripening. Molecular Plant-Microbe Interactions, 2015, 28.11: 1167-1180.Type
info:eu-repo/semantics/articlePublisher version
http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-02-15-0039-RSubject
Abstract
Mature grapevine berries at the harvesting stage (MB) are very susceptible to the gray mold fungus Botrytis cinerea, while veraison berries (VB) are not. We conducted simultaneous microscopic and transcriptomic analyses ... [+]
Mature grapevine berries at the harvesting stage (MB) are very susceptible to the gray mold fungus Botrytis cinerea, while veraison berries (VB) are not. We conducted simultaneous microscopic and transcriptomic analyses of the pathogen and the host to investigate the infection process developed by B. cinerea on MB versus VB, and the plant defense mechanisms deployed to stop the fungus spreading. On the pathogen side, our genome-wide transcriptomic data revealed that B. cinerea genes upregulated during infection of MB are enriched in functional categories related to necrotrophy, such as degradation of the plant cell wall, proteolysis, membrane transport, reactive oxygen species (ROS) generation, and detoxification. Quantitative-polymerase chain reaction on a set of representative genes related to virulence and microscopic observations further demonstrated that the infection is also initiated on VB but is stopped at the penetration stage. On the plant side, genome-wide transcriptomic analysis and metabolic data revealed a defense pathway switch during berry ripening. In response to B. cinerea inoculation, VB activated a burst of ROS, the salicylate-dependent defense pathway, the synthesis of the resveratrol phytoalexin, and cell-wall strengthening. On the contrary, in infected MB, the jasmonate-dependent pathway was activated, which did not stop the fungal necrotrophic process. [-]
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Molecular Plant-Microbe Interactions, November 2015, Volume 28, Number 11 Pages 1167-1180Rights
Copyright © 2015 The American Phytopathological Society
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