A method to quantify several tyrosine kinase inhibitors in plasma by micellar liquid chromatography and validation according to the european medicines agency guidelines
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Other documents of the author: Garrido Cano, Iris; García García, Aurelio; Vives-Peris, Vicente; Ochoa Aranda, Enrique; Esteve-Romero, Josep
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comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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http://dx.doi.org/10.1016/j.talanta.2015.07.078 |
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Title
A method to quantify several tyrosine kinase inhibitors in plasma by micellar liquid chromatography and validation according to the european medicines agency guidelinesAuthor (s)
Date
2015-11Publisher
ElsevierBibliographic citation
GARRIDO-CANO, Iris, et al. A method to quantify several tyrosine kinase inhibitors in plasma by micellar liquid chromatography and validation according to the European Medicines Agency guidelines. Talanta, 2015, vol. 144, p. 1287-1295.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0039914015302046Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
A procedure based on micellar liquid chromatography has been developed to monitor five tyrosine kinase inhibitors in plasma, prescribed against several kinds of cancer: erlotinib, imatinib, sunitinib, sorafenib and ... [+]
A procedure based on micellar liquid chromatography has been developed to monitor five tyrosine kinase inhibitors in plasma, prescribed against several kinds of cancer: erlotinib, imatinib, sunitinib, sorafenib and lapatinib. The sample was diluted in a micellar solution and directly injected, thus clean-up steps were not required. The analytes were resolved without interferences in <20 min using a C18 column and a mobile phase of 0.13 M SDS-4% 1-butanol, buffered at pH 3.5, running under isocratic mode at 1 mL/min. The detection was performed by UV–visible absorbance, using a wavelength program to maximize the signal-to-noise ratio. The method was validated following the guideline of the European Medicines Agency in terms of: selectivity, calibration range (0.05–5 μg/mol), linearity (r2>0.990), limit of detection (15–35 ng/mL), carry-over effect, accuracy (−10.4 to +11.0%), precision (<9.2%), matrix effect, robustness (<8.4%) and stability. The procedure is rapid, easy-to-handle, uses a low amount of toxic chemical provide reliable results. Finally, the method was successfully used to analyze the studied tyrosine kinase inhibitors in plasma from cancer patients. [-]
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Talanta Volume 144, 1 November 2015Rights
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