Quantification of Callose Deposition in Plant Leaves
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Other documents of the author: Scalschi, Loredana; Llorens, Eugenio; Camañes, Gemma; Pastor, Victoria; Fernández Crespo, Emma; Flors, Victor; García Agustín, Pilar; Vicedo, Begonya
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Title
Quantification of Callose Deposition in Plant LeavesAuthor (s)
Date
2015-10Publisher
Bio-protocol LLCISSN
2331-8325Bibliographic citation
SCALSCHI, Loredana, et al. Quantification of Callose Deposition in Plant Leaves. 2015.Type
info:eu-repo/semantics/articlePublisher version
http://www.bio-protocol.org/e1610Abstract
Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is
widespread in higher plants. Callose is involved in multiple aspects of plant growth and
development. It is synthetized in plants at the cell ... [+]
Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is
widespread in higher plants. Callose is involved in multiple aspects of plant growth and
development. It is synthetized in plants at the cell plate during cytokinesis, in several stages
during pollen development and is deposited at plasmodesmata to regulate the cell-to-cell
movement of molecules. Moreover, it is produced in response to multiple biotic and abiotic
stresses (Chen and Kim, 2009). Callose is considered to act as a physical barrier by
strengthening the plant cell well to slow pathogen infection and to contribute to the plant’s
innate immunity. Thus the callose staining method is useful to quantify activity of plant immunity.
In addition, this staining can be used to visualize structures in plant tissue, where the callose
may be implied whether during the development of plants or response against pathogen
infection. This method is based on the use of methyl blue which reacts with (13)--glucans to
give a brilliant yellow fluorescence in UV light. Moreover, calcofluor stains chitin present in
fungal cell membranes and also binds to cellulose at locations where the cuticle is damaged. [-]
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Bio-protocol 5(19), 2015Rights
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