Application of liquid chromatography/mass spectrometry in assessment of potential use of azadirachtins (TreeAzin ™ ) against Asian longhorned beetle
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Otros documentos de la autoría: Beltrán Iturat, Eduardo; Ibañez Martinez, Maria; GRACIA LOR, EMMA; Sancho, Juan V; Hernandez, Felix; Thompson, D. G.
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Título
Application of liquid chromatography/mass spectrometry in assessment of potential use of azadirachtins (TreeAzin ™ ) against Asian longhorned beetleAutoría
Fecha de publicación
2014-08xmlui.dri2xhtml.METS-1.0.item-edition
PostprintEditor
Royal Society of ChemistryCita bibliográfica
BELTRÁN ITURAT, E.; IBÁÑEZ MARTÍNEZ, M.; GRACIA LOR, E.; SANCHO LLOPIS, J. V.; HERNÁNDEZ HERNÁNDEZ, F.; THOMPSON, D. G. Application of liquid chromatography/mass spectrometry in assessment of potential use of azadirachtins (TreeAzin ™ ) against Asian longhorned beetle. Analytical Methods, n. 6 (2014) pp. 8063-8071Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/c4ay01553c#!divAbstractPalabras clave / Materias
Resumen
Azadirachtins are natural triterpenoid compounds derived from Neem tree extracts with potential for use as systemic insecticides against invasive wood-boring insect pests. In this work, a sensitive and selective ... [+]
Azadirachtins are natural triterpenoid compounds derived from Neem tree extracts with potential for use as systemic insecticides against invasive wood-boring insect pests. In this work, a sensitive and selective analytical method has been developed for the simultaneous determination of azadirachtin A and azadirachtin B (3-tigloylazadirachtol) in foliage and twigs of various tree species. Samples were mixed with C18 and primary-secondary amine (PSA), and extracted with acetonitrile. Then, an aliquot of the raw
extract was 10-fold diluted with water and directly analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The method was validated in foliage and twig matrices of four different tree species (London Plane Tree, Red/Freemani Maple, Norway Maple and Sugar Maple) that are known hosts of the exotic invasive insect pest
– Asian Longhorn Beetle (ALB). Analytical results for replicate (N = 5) samples, fortified at 0.01, 0.1 and 1 mg kg , showed good recoveries (86 – 119%) and precision (<20%
RSD). The methodology was successfully applied to the analysis of 200 samples taken from a field experiment designed to investigate uptake, translocation and expression of azadirachtins in representative high-value urban trees followed by stem injection with TreeAzinTM
™ [-]
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Analytical Methods, n. 6 (2014)Derechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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