Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads
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Other documents of the author: Izquierdo Henríquez, Diana F.; Barbosa, Oveimar; Burguete, M. Isabel; Lozano, Pedro; Luis, Santiago V.; Fernandez Lafuente, Roberto; Garcia-Verdugo, Eduardo
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comunitat-uji-handle2:10234/7053
comunitat-uji-handle3:10234/8639
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Title
Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beadsAuthor (s)
Date
2013-12Publisher
Royal Society of ChemistryBibliographic citation
IZQUIERDO, Diana F., et al. Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads. RSC Advances, 2014, 4.12: 6219-6225.Type
info:eu-repo/semantics/articleSubject
Abstract
Lipase B from Candida antarctica (CALB) is able to catalyze C–C bond formation. After immobilization onto a hydrophobic PS-DVB support, the activity increases when compared to that of the soluble or tan – the commer ... [+]
Lipase B from Candida antarctica (CALB) is able to catalyze C–C bond formation. After immobilization onto a hydrophobic PS-DVB support, the activity increases when compared to that of the soluble or tan – the commercially available Novozyme 435 (being up to 6 fold more active). Our results show that although this activity is not related to the catalytic group, the promiscuous activity of CALB may be tuned via immobilization. In addition, we have show that the secondary structure of both immobilized enzymes is quite different, using FT-ATR-IR spectroscopy. [-]
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RSC Adv., 2014,4Rights
© The Royal Society of Chemistry 2014
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