Fine Tuning of Reactive Oxygen Species Homeostasis Regulates Primed Immune Responses in Arabidopsis
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Otros documentos de la autoría: Pastor, Victoria; Luna Diez, Estrella; Ton, Jurriaan; Cerezo García, Miguel; García Agustín, Pilar; Flors, Victor
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http://dx.doi.org/10.1094/MPMI-04-13-0117-R |
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Título
Fine Tuning of Reactive Oxygen Species Homeostasis Regulates Primed Immune Responses in ArabidopsisAutoría
Fecha de publicación
2013-11Editor
The American Phytopathological Society; International Society for Molecular Plant-Microbe InteractionsISSN
0894-0282Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-04-13-0117-RResumen
Selected stimuli can prime th
e plant immune system for a
faster and stronger defense reaction to pathogen attack.
Pretreatment of
Arabidopsis
with the chemical agent
β
-
aminobutyric acid (BABA) augmented ... [+]
Selected stimuli can prime th
e plant immune system for a
faster and stronger defense reaction to pathogen attack.
Pretreatment of
Arabidopsis
with the chemical agent
β
-
aminobutyric acid (BABA) augmented H
2
O
2
and callose
production after induction with the pathogen-associated
molecular pattern (PAMP) chitosan, or inoculation with
the necrotrophic fungus
Plectosphaerella cucumerina.
How-
ever, BABA failed to prime H
2
O
2
and callose production
after challenge with the bacterial PAMP Flg22.
Analysis of
Arabidopsis
mutants in reactive oxygen species (ROS) pro-
duction (
rbohD
) or ROS scavenging (
pad2
,
vtc1
, and
cat2
)
suggested a regulatory role for ROS homeostasis in prim-
ing of chitosan- and
P. c u c u m e r i n a
-inducible callose and
ROS. Moreover,
rbohD
and
pad2
were both impaired in
BABA-induced resistance against
P. cucumerina
. Gene ex-
pression analysis revealed direct induction of
NADPH/res-
piratory burst oxidase protein D
(
RBOHD
),
γ
-glutamylcys-
teine synthetase 1
(
GSH1
), and
vitamin C defective 1
(
VTC1
)
genes after BABA treatment. Conversely,
ascorbate peroxi-
dase 1
(
APX1
) transcription was repressed by BABA after
challenge with chitosan or
P. cucumerina
, probably to pro-
vide a more oxidized environment in the cell and facilitate
augmented ROS accumulation.
Measuring ratios between
reduced and oxidized glutathione confirmed that augmented
defense expression in primed plants is associated with a
more oxidized cellular status. Together, our data indicate
that an altered ROS equilibrium is required for augmented
defense expression in primed plants. [-]
Publicado en
Molecular Plant-Microbe Interactions, vol. 26, n. 11, p. 1334-1344Derechos de acceso
© 2013 The American Phytopathological Society
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