Listar por autoría "bda15c25-3736-490c-aa3f-1b2499e72a6b"
Mostrando ítems 21-40 de 48
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Inhibition of the human respiratory syncytial virus small hydrophobic protein and structural variations in a bicelle environment
Li, Yan; To, Janet; Verdiá Báguena, Carmen; Dossena, Silvia; Surya, Wahyu; Huang, Mei; Paulmichl, Markus; Liu, Ding Xiang; Aguilella, Vicente; Torres, Jaume American Society for Microbiology (2014)The small hydrophobic (SH) protein is a 64-amino-acid polypeptide encoded by the human respiratory syncytial virus (hRSV). SH protein has a single α-helical transmembrane (TM) domain that forms pentameric ion channels. ... -
Insights on the permeability of wide protein channels: measurement and interpretation of ion selectivity
Aguilella, Vicente; Queralt-Martín, María; Aguilella-Arzo, Marcel; Alcaraz, Antonio Royal Society of Chemistry (2012-10-11)Ion channels are hollow proteins that have evolved to exhibit discrimination between charged solutes. This property, known as ion selectivity is critical for several biological functions. By using the bacterial porin OmpF ... -
Ion channel activity of the CSFV p7 viroporin in surrogates of the ER lipid bilayer
Largo, Eneko; Verdiá Báguena, Carmen; Aguilella, Vicente; Nieva, José L.; Alcaraz, Antonio Elsevier (2016-01)Viroporins comprise a family of non-structural proteins that play significant and diverse roles during the replication cycle of many animal viruses. Consequently, they have become promising targets for inhibitory drug and ... -
Ion selectivity of a biological channel at high concentration ratio: insights on small ion diffusion and binding
López Peris, María Lidón; Aguilella-Arzo, Marcel; Aguilella, Vicente; Alcaraz, Antonio American Chemical Society (2009)The ionic selectivity of the bacterial porin OmpF has been investigated in several chloride salts of alkali metal cations under a wide range of concentration ratios. We report a novel way of studying the several factors ... -
Ion Transport in Confined Geometries below the Nanoscale: Access Resistance Dominates Protein Channel Conductance in Diluted Solutions
Alcaraz, Antonio; López Peris, María Lidón; Queralt-Martín, María; Aguilella, Vicente (2017)Synthetic nanopores and mesoscopic protein channels have common traits like the importance of electrostatic interactions between the permeating ions and the nanochannel. Ion transport at the nanoscale occurs under confinement ... -
Linearity, saturation and blocking in a large multiionic channel: divalent cation modulation of the OmpF porin conductance
García Giménez, Elena; López Peris, María Lidón; Aguilella, Vicente; Alcaraz, Antonio Elsevier (2011-01)Measurement of unitary conductance is a fundamental step in the characterization of a protein ion channelpermeabilizing a membrane. We study here the effect of salts of divalent cations on the OmpF channelconductance ... -
Lipid charge regulation of non-specific biological ion channels
Aguilella, Vicente; Verdiá Báguena, Carmen; Alcaraz, Antonio Royal Society of Chemistry (2014)Ion channels are specialized proteins that enable the movement of charges through otherwise impermeable lipidic membranes. Their action is essential in living organisms facilitating electric signaling, muscle contraction ... -
MERS coronavirus envelope protein has a single transmembrane domain that forms pentameric ion channels
Surya, Wahyu; Li, Yan; Verdiá Báguena, Carmen; Aguilella, Vicente; Torres, Jaume Elsevier (2015-04)The Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly identified pathogen able of human transmission that causes a mortality of almost 40%. As in the case of SARS-CoV, MERS virus lacking E protein represents ... -
Molecular Characterization of the Viroporin Function of Foot-and-Mouth Disease Virus Nonstructural Protein 2B
Gladue, Douglas; Largo, Eneko; de la Arada, Igor; Aguilella, Vicente; Alcaraz, Antonio; Arrondo, Jose Luis; Holinka, L.G.; Brocchi, Emiliana; Ramirez-Medina, Elizabeth; Vuono, Elizabeth; Berggren, Keith; Carrillo, C.; Nieva, Jose L; Borca, Manuel V. American Society for Microbiology (2018-12)Nonstructural protein 2B of foot-and-mouth disease (FMD) virus (FMDV) is comprised of a small, hydrophobic, 154-amino-acid protein. Structure-function analyses demonstrated that FMDV 2B is an ion channel-forming protein. ... -
Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses
Largo, Eneko; Gladue, Douglas; Torralba, Johana; Aguilella, Vicente; Alcaraz, Antonio; Borca, Manuel V.; Nieva, José L. Elsevier (2018-05)Permeabilization of the Endoplasmic Reticulum (ER) is instrumental in the progression of host-cell infection by many viral pathogens. We have described that permeabilization of ER model membranes by the pore-forming domain ... -
PEG Equilibrium Partitioning in the α-Hemolysin Channel: Neutral Polymer Interaction with Channel Charges
Aguilella-Arzo, Marcel; Aguilella, Vicente American Chemical Society (2021-02-08)We study the interaction of neutral polyethylene glycol (PEG) molecules of different molecular weights (MWs) with the charged residues of the α-hemolysin channel secreted by Staphylococcus aureus. Previously reported ... -
Probing Tubulin-Blocked State of VDAC by Varying Membrane Surface Charge
Gurnev, Philip A.; Queralt-Martín, María; Aguilella, Vicente; Rostovtseva, Tatiana K. Biophysical Society (2012-05) -
Protein Ion Channels as Molecular Ratchets. Switchable Current Modulation in Outer Membrane Protein F Porin Induced by Millimolar La3+ Ions
Verdiá Báguena, Carmen; Queralt-Martín, María; Aguilella, Vicente; Alcaraz, Antonio American Chemical Society (2012)The quest for innovative tunable nanodevices has mainly focused on switches that modulate their properties through engineered conformational changes. We propose here an alternative route that takes advantage of the crucial ... -
Rectification properties and pH-dependent selectivity of meningococcal class 1 porin
Cervera, Javier; Komarov, Alexander G.; Aguilella, Vicente (2008)We studied the current rectification properties and selectivity of class 1 porin (PorA) from Neisseria meningitidis (strain H44/76 A3A4) reconstituted in planar lipid membranes varying salt concentrations and pH. PorA ... -
Relevance of Viroporin Ion Channel Activity on Viral Replication and Pathogenesis
Nieto Torres, José L.; Verdiá Báguena, Carmen; Castaño Rodríguez, Carlos; Aguilella, Vicente; Enjuanes, Luis MDPI (2015-07)Modification of host-cell ionic content is a significant issue for viruses, as several viral proteins displaying ion channel activity, named viroporins, have been identified. Viroporins interact with different cellular ... -
Role of Severe Acute Respiratory Syndrome Coronavirus Viroporins E, 3a, and 8a in Replication and Pathogenesis
Castaño Rodríguez, Carlos; Honrubia, José M.; Gutiérrez-Álvarez, Javier; De Diego, Marta L.; Nieto Torres, José L.; Jiménez Guardeño, José M.; Regla Nava, José A.; Fernández Delgado, Raúl; Verdiá Báguena, Carmen; Queralt-Martín, María; Kochan, Grazyna; Perlman, Stanley; Aguilella, Vicente; Sola, Isabel; Enjuanes, Luis American Society for Microbiology (2018)Viroporins are viral proteins with ion channel (IC) activity that play an important role in several processes, including virus replication and pathogenesis. While many coronaviruses (CoVs) encode two viroporins, severe ... -
SARS-CoV-2 accessory protein 7b forms homotetramers in detergent
Surya, Wahyu; Queralt-Martin, Raquel; Mu, Yuguang; Aguilella, Vicente; Torres, Jaume BioMed Central (BMC) (2022-11)A global pandemic is underway caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 genome, like its predecessor SARS-CoV, contains open reading frames that encode accessory proteins ... -
Scaling Behavior of Ionic Transport in Membrane Nanochannels
Queralt-Martín, María; López Peris, María Lidón; Aguilella-Arzo, Marcel; Aguilella, Vicente; Alcaraz, Antonio American Chemical Society (2018)Ionic conductance in membrane channels exhibits a power-law dependence on electrolyte concentration (G ∼ c α ). The many scaling exponents, α, reported in the literature usually require detailed interpretations ... -
Selectivity of Protein Ion Channels and the Role of Buried Charges. Analytical Solutions, Numerical Calculations, and MD Simulations
García Giménez, Elena; Alcaraz, Antonio; Aguilella-Arzo, Marcel; Aguilella, Vicente American Chemical Society (2015-06)The preference of large protein ion channels for cations or anions is mainly determined by the electrostatic interactions of mobile ions with charged residues of the protein. Here we discuss the widely spread paradigm that ... -
Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome
Nieto Torres, José L.; Verdiá Báguena, Carmen; Jiménez Guardeño, José M.; Regla Nava, José A.; Castaño Rodríguez, Carlos; Fernández Delgado, Raúl; Torres, Jaume; Aguilella, Vicente; Enjuanes, Luis Elsevier (2015-11)Severe acute respiratory syndrome coronavirus (SARS-CoV) envelope (E) protein is a viroporin involved in virulence. E protein ion channel (IC) activity is specifically correlated with enhanced pulmonary damage, edema ...