Listar por autoría "38419e93-e6a9-48c8-9dfe-a4db00ad5534"
Mostrando ítems 1-20 de 48
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A Multidisciplinary Approach to Sexual Behavior Profiles: The SEX360 Model
García Cruz, Eduard; Monguet, Josep Maria; Marre, Diana; González, Mònica; Peraza, Maria Fernanda; Sánchez, Carme; Suso-Ribera, Carlos; Trejo, Álex; Alcaraz, Antonio Springer Verlag (2017)The diversity of sexual behaviors is driven by multiple determinants, including physiological, cultural, educational, and sociological factors. However, the definition of sexual behavior profiles has been barely addressed ... -
African Swine Fever Virus Gene B117L Encodes a Small Protein Endowed with Low-pH-Dependent Membrane Permeabilizing Activity
Gladue, Douglas; Gómez Lucas, Lidia; Largo, Eneko; Velazquez-Salinas, Lauro; Ramirez-Medina, Elizabeth; Torralba, Johana; Queralt-Martin, Maria; Alcaraz, Antonio; Nieva, Jose L; Borca, Manuel V. American Society for Microbiology (2023-05-22)African swine fever virus (ASFV) is causing a devastating pandemic in domestic and wild swine in Central Europe to East Asia, resulting in economic losses for the swine industry. The virus contains a large double-stranded ... -
Amphiphilic COSAN and I2-COSAN crossing synthetic lipid membranes: planar bilayers and liposomes
Verdiá Báguena, Carmen; Alcaraz, Antonio; Aguilella, Vicente; Cioran, Ana M.; Tachikawa, Shoji; Nakamura, Hiroyuki; Teixidor, Francesc; Viñas, Clara Royal Society of Chemistry (2014-03)The boron-rich cobaltabisdicarbollide (COSAN) and its 8,80-I2 derivative (I2-COSAN), both of purely inorganic nature, are shown to cross through synthetic lipid membranes. These results reveal unexpected properties at the ... -
Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge
Verdiá Báguena, Carmen; Nieto Torres, José L.; Alcaraz, Antonio; De Diego, Marta L.; Enjuanes, Luis; Aguilella, Vicente Elsevier (2013-05-18)A partial characterization of the ion channels formed by the SARS coronavirus (CoV) envelope (E) protein was previously reported (C. Verdiá-Báguena et al., 2012 [12]). Here, we provide new significant insights on the ... -
Bioelectrical Signals and Ion Channels in the Modeling of Multicellular Patterns and Cancer Biophysics
Cervera, Javier; Alcaraz, Antonio; Mafe, Salvador Nature Publishing Group (2016)Bioelectrical signals and ion channels are central to spatial patterns in cell ensembles, a problem of fundamental interest in positional information and cancer processes. We propose a model for electrically connected ... -
Biphasic concentration patterns in ionic transport under nanoconfinement revealed in steady-state and time-dependent properties
Queralt-Martín, María; Perez-Grau, Jose J.; Alvero González, Laidy Maidel; Perini, Deborah Aurora; Cervera, Javier; Aguilella, Vicente; Alcaraz, Antonio American Institute of Physics (2023-02-08)Ion permeation across nanoscopic structures differs considerably from microfluidics because of strong steric constraints, transformed solvent properties, and charge-regulation effects revealed mostly in diluted solutions. ... -
Coronavirus E protein forms ion channels with functionally and structurally-involved membrane lipids
Aguilella, Vicente; Alcaraz, Antonio; Verdiá Báguena, Carmen; Nieto Torres, José L.; De Diego, Marta L.; Torres, Jaume; Enjuanes, Luis Elsevier (2012-10)Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV ... -
Dielectric saturation of water in a membrane protein channel
Aguilella-Arzo, Marcel; Andrio, Andreu; Aguilella, Vicente; Alcaraz, Antonio Royal Society of Chemistry (2009)Water molecules in confined geometries like nanopores and biological ion channels exhibit structural and dynamical properties very different from those found in free solution. Protein channels that open aqueous pores ... -
Diffusion, exclusion, and specific binding in a large channel: A study of OmpF selectivity inversion
Alcaraz, Antonio; Nestorovich E.M.; López Peris, María Lidón; García Giménez, Elena; Bezrukov S.M.; Aguilella, Vicente Elsevier (2009)We find that moderate cationic selectivity of the general bacterial porin OmpF in sodium and potassium chloride solutions is inversed to anionic selectivity in concentrated solutions of barium, calcium, nickel, and magnesium ... -
Directional ion selectivity in a biological nanopore with bipolar structure
García Giménez, Elena; Alcaraz, Antonio; Aguilella, Vicente; Ramírez, Patricio Elsevier (2009)Ion transport features of a biological nanopore, the bacterial porin OmpF from Escherichia coli, have been investigated by patch-clamp experiments performed at the single channel level. Membrane potential measurements done ... -
Divalent cations reduce the pH sensitivity of OmpF channel inducing the pKa shift of key acidic residues
Queralt-Martín, María; Alcaraz, Antonio; García Giménez, Elena; Mafe, Salvador RSC Publishing (2010-11-03)In contrast to the highly-selective channels of neurophysiology employing mostly the exclusion mechanism, different factors account for the selectivity of large channels. Elucidation of these factors is essential for ... -
Divalent Metal Ion Transport across Large Biological Ion Channels and Their Effect on Conductance and Selectivity
García Giménez, Elena; Alcaraz, Antonio; Aguilella, Vicente Hindawi Publishing Corporation (2012-07)Electrophysiological characterization of large protein channels, usually displaying multi-ionic transport and weak ion selectivity, is commonly performed at physiological conditions (moderate gradients of KCl solutions at ... -
Dynorphin A induces membrane permeabilization by formation of proteolipidic pores. Insights from electrophysiology and computational simulations
Perini, Deborah Aurora; Aguilella-Arzo, Marcel; Alcaraz, Antonio; Perálvarez-Marín, Alex; Queralt-Martín, María Elsevier (2021-12-16)Dynorphins are endogenous neuropeptides that function as ligands for the κ-opioid receptor. In addition to opioid activity, dynorphins can induce several pathological effects such as neurological dysfunctions and cell ... -
Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel
Kalu, Nnanya; Alcaraz, Antonio; Yamini, Goli; Mombem Abolfath, Sanaz; Lucas, Laura; Kenney, Clare; Aguilella, Vicente; Nestorovich, Ekaterina M. Wiley (2017-11)Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ by ... -
Electrical pumping of potassium ions against an external concentration gradient in a biological ion channel
Aguilella, Vicente; Alcaraz, Antonio; Ramírez, Patricio; García Giménez, Elena; Mafe, Salvador; Queralt-Martín, María AIP Publishing (2013-07-22)We show experimentally and theoretically that significant currents can be obtained with a biological ion channel, the OmpF porin of Escherichia coli, using zero-average potentials as driving forces. The channel rectifying ... -
Entropy–enthalpy compensation at the single protein level: pH sensing in the bacterial channel OmpF
Alcaraz, Antonio; Queralt-Martín, María; Verdiá Báguena, Carmen; Aguilella, Vicente; Mafe, Salvador The Royal Society of Chemistry (2014)The pH sensing mechanism of the OmpF channel operates via ligand modification: increasing acidity induces the replacement of cations with protons in critical binding sites decreasing the channel conductance. Aside from the ... -
Excess white noise to probe transport mechanisms in a membrane channel
Queralt-Martín, María; López Peris, María Lidón; Alcaraz, Antonio American Physical Society (2015)Current fluctuation analysis has been successfully used over the years to investigate the physical properties of different systems. Here, we perform single-channel time-resolved current experiments in a protein channel ... -
Experimental and computational biophysics to identify vasodilator drugs targeted at TRPV2 using agonists based on the probenecid scaffold
Catalina-Hernández, Èric; López-Martín, Mario; Masnou-Sánchez, David; Martins, Marco; Lorenz Fonfria, Victor A.; Jiménez-Altayó, Francesc; Hellmich, Ute A.; Inada, Hitoshi; Alcaraz, Antonio; Furutani, Yuji; Nonell-Canals, Alfons; Vázquez-ibar, José Luis; Domene, Carmen; Gaudet, Rachelle; Perálvarez-Marín, Alex Elsevier (2023-12-29)TRP channels are important pharmacological targets in physiopathology. TRPV2 plays distinct roles in cardiac and neuromuscular function, immunity, and metabolism, and is associated with pathologies like muscular dystrophy ... -
Experimental demonstration of charge inversion in a protein channel in the presence of monovalent cations
López Peris, María Lidón; Queralt-Martín, María; Alcaraz, Antonio Elsevier (2014-11)Charge inversion takes place when interfacial charges attract counterions in excess of their own nominal charge. To date, this phenomenon has been reported in a variety of membrane channel systems in the presence of ... -
Fluctuation-Driven Transport in Biological Nanopores. A 3D Poisson–Nernst–Planck Study
Aguilella-Arzo, Marcel; Queralt-Martín, María; López, María Lidón; Alcaraz, Antonio MDPI (2017)Living systems display a variety of situations in which non-equilibrium fluctuations couple to certain protein functions yielding astonishing results. Here we study the bacterial channel OmpF under conditions similar to ...