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dc.contributor.authorMishra, Swati Dubey
dc.contributor.authorBose, Devasish
dc.contributor.authorShukla, S. K.
dc.contributor.authorDurgbanshi, Abhilasha
dc.contributor.authorEsteve-Romero, Josep
dc.identifier.citationMISHRA, S. D.; BOSE, D.; SHUKLA, S. K.; DURGBANSHI, A.; ESTEVE ROMERO, J. Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography. Analytical Methods, issue 7 (2013), p. 1747-1754ca_CA
dc.description.abstractNux-vomica is a tropical plant that contains two ergot alkaloids i.e. strychnine and brucine which have pharmaceutical and toxic properties. In some places, for example in India, Nux-vomica is used in ayurvedic preparations in homeopathic medicine. As both compounds are toxic monitoring is required. In this work a method for the chromatographic determination of strychnine and brucine in various herbal/ayurvedic sample preparations, homeopathic medicines, containing Nux-vomica, seeds of Nux-vomica as well as in spiked serum and real urine samples are described. The method involves a direct injection micellar liquid chromatography technique that was optimised under ICH guidelines. Optimum conditions are a mobile phase composed by 0.10 M SDS-pentanol 4% (v/v)-NaH2PO4 buffered at pH 3 with UV detection at 258 nm, flow rate of 1 mL min−1, and temperature 25 °C. Under these conditions strychnine and brucine were analyzed in 15 and 11 min, respectively. Validation studies using ICH guidelines were performed to show that the proposed method was linear (r2 > 0.9997), with LODs (0.18 and 0.09) and LOQs (0.69 and 0.35), for strycnine and brucine, respectively. Repeatability intra- and inter-day was below of 2.50. The developed method can be useful for analyzing strychnine and brucine for quality control and other related forensic and clinical casesca_CA
dc.format.extent8 p.ca_CA
dc.publisherRoyal Society of Chemistryca_CA
dc.relation.isPartOfAnalytical Methods, issue 7 (2013)ca_CA
dc.subjectLiquid chromatographyca_CA
dc.subjectHomeopathic medicineca_CA
dc.subjectPharmaceutical propertiesca_CA
dc.subjectToxic propertiesca_CA
dc.titleMonitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatographyca_CA

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