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Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography
dc.contributor.author | Mishra, Swati Dubey | |
dc.contributor.author | Bose, Devasish | |
dc.contributor.author | Shukla, S. K. | |
dc.contributor.author | Durgbanshi, Abhilasha | |
dc.contributor.author | Esteve-Romero, Josep | |
dc.date.accessioned | 2014-06-11T12:45:28Z | |
dc.date.available | 2014-06-11T12:45:28Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | MISHRA, S. D.; BOSE, D.; SHUKLA, S. K.; DURGBANSHI, A.; ESTEVE ROMERO, J. Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography. Analytical Methods, issue 7 (2013), p. 1747-1754 | ca_CA |
dc.identifier.uri | http://hdl.handle.net/10234/94625 | |
dc.description.abstract | Nux-vomica is a tropical plant that contains two ergot alkaloids i.e. strychnine and brucine which have pharmaceutical and toxic properties. In some places, for example in India, Nux-vomica is used in ayurvedic preparations in homeopathic medicine. As both compounds are toxic monitoring is required. In this work a method for the chromatographic determination of strychnine and brucine in various herbal/ayurvedic sample preparations, homeopathic medicines, containing Nux-vomica, seeds of Nux-vomica as well as in spiked serum and real urine samples are described. The method involves a direct injection micellar liquid chromatography technique that was optimised under ICH guidelines. Optimum conditions are a mobile phase composed by 0.10 M SDS-pentanol 4% (v/v)-NaH2PO4 buffered at pH 3 with UV detection at 258 nm, flow rate of 1 mL min−1, and temperature 25 °C. Under these conditions strychnine and brucine were analyzed in 15 and 11 min, respectively. Validation studies using ICH guidelines were performed to show that the proposed method was linear (r2 > 0.9997), with LODs (0.18 and 0.09) and LOQs (0.69 and 0.35), for strycnine and brucine, respectively. Repeatability intra- and inter-day was below of 2.50. The developed method can be useful for analyzing strychnine and brucine for quality control and other related forensic and clinical cases | ca_CA |
dc.format.extent | 8 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Royal Society of Chemistry | ca_CA |
dc.relation.isPartOf | Analytical Methods, issue 7 (2013) | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/CNE/1.0/ | * |
dc.subject | Strychnine | ca_CA |
dc.subject | Brucine | ca_CA |
dc.subject | Liquid chromatography | ca_CA |
dc.subject | Nux-vomica | ca_CA |
dc.subject | Homeopathic medicine | ca_CA |
dc.subject | Pharmaceutical properties | ca_CA |
dc.subject | Toxic properties | ca_CA |
dc.title | Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1039/C3AY26014C | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://pubs.rsc.org/en/content/articlelanding/2013/AY/c3ay26014c#!divAbstract | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
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