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Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice
dc.contributor.author | Baliño, Pablo | |
dc.contributor.author | Monferrer Sales, Lidón | |
dc.contributor.author | Pastor Medall, Raúl | |
dc.contributor.author | González Aragón, Carlos Manuel | |
dc.date.accessioned | 2013-07-10T18:41:23Z | |
dc.date.available | 2013-07-10T18:41:23Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0014-4886 | |
dc.identifier.issn | 1090-2430 | |
dc.identifier.uri | http://hdl.handle.net/10234/70142 | |
dc.description.abstract | Calcium (Ca2+) has been characterized as one of the most ubiquitous, universal and versatile intracellular signaling molecules responsible for controlling numerous cellular processes. Ethanol-induced effects on Ca2+ distribution and flux have been widely studied in vitro, showing that acute ethanol administration can modulate intracellular Ca2+ concentrations in a dose dependent manner. In vivo, the relationship between Ca2+ manipulation and the corresponding ethanol-induced behavioral effects have focused on Ca2+ flux through voltage-gated Ca2+ channels. The present study investigated the role of inward Ca2+ currents in ethanol-induced psychomotor effects (stimulation and sedation) and ethanol intake. We studied the effects of the fast Ca2+ chelator, BAPTA-AM, on ethanol-induced locomotor activity and the sedative effects of ethanol. Swiss (RjOrl) mice were pretreated with BAPTA-AM (0–10 mg/kg) 30 min before an ethanol (0–4 g/kg) challenge. Our results revealed that pretreatment with BAPTA-AM prevented locomotor stimulation produced by ethanol without altering basal locomotion. In contrast, BAPTA-AM reversed ethanol-induced hypnotic effects. In a second set of experiments, we investigated the effects of intracellular Ca2+ chelation on ethanol intake. Following a drinking-in-the-dark methodology, male C57BL/6J mice were offered 20% v/v ethanol, tap water, or 0.1% sweetened water. The results of these experiments revealed that BAPTA-AM pretreatment (0–5 mg/kg) reduced ethanol consumption in a dose-dependent manner while leaving water and sweetened water intake unaffected. Our findings support the role of inward Ca2+ currents in mediating different behavioral responses induced by ethanol. Our results are discussed together with data indicating that ethanol appears to be more sensitive to intracellular Ca2+ manipulations than other psychoactive drugs. | ca_CA |
dc.format.extent | 8 p. | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Experimental Neurology Volume 234, Issue 2, April 2012 | ca_CA |
dc.rights | © 2012 Elsevier Inc. All rights reserved. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Ethanol | ca_CA |
dc.subject | Calcium | ca_CA |
dc.subject | BAPTA-AM | ca_CA |
dc.subject | Locomotor activity | ca_CA |
dc.subject | Ethanol administration | ca_CA |
dc.subject | Righting reflex | ca_CA |
dc.title | Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1016/j.expneurol.2012.01.016 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0014488612000362 | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
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