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dc.contributor.authorSabater Muñoz, Beatriz
dc.contributor.authorPascual Ruiz, Sara
dc.contributor.authorGómez Martínez, María A.
dc.contributor.authorJacas Miret, Josep Anton
dc.contributor.authorHurtado Ruiz, Mónica
dc.date.accessioned2013-07-09T12:33:35Z
dc.date.available2013-07-09T12:33:35Z
dc.date.issued2012
dc.identifier.citationSabater-Muñoz, B., Pascual-Ruiz, S., Gómez-Martínez, M. A., Jacas, J. A., & Hurtado, M. A. (2012). Isolation and characterization of polymorphic microsatellite markers in Tetranychus urticae and cross amplification in other Tetranychidae and Phytoseiidae species of economic importance. Experimental and Applied Acarology, 57(1), 37-51.ca_CA
dc.identifier.issn0168-8162
dc.identifier.issn1572-9702
dc.identifier.urihttp://hdl.handle.net/10234/70000
dc.description.abstractTetranychus urticae Koch is a cosmopolitan phytophagous mite considered as the most polyphagous species among spider mites. Population genetic studies using molecular markers such as microsatellites have proven to be extremely informative to address questions about population structure, phylogeography and host preferences. The aim of this study was to increase the available molecular tools to gain insight into the genetic structure of T. urticae populations of citrus orchards, which might help in their management. Five microsatellite DNA libraries were developed using probes with the motifs CT, CTT, GT and CAC following the FIASCO protocol. Positive clones, those that included the insert with the microsatellite, were detected using the PIMA-PCR technique. Combinations of primers were designed on 22 out of 32 new microsatellites loci and their polymorphism was tested in four populations sampled along the eastern coast of Spain. Eleven successful amplifications were obtained. Cross amplification was tested in the tetranychids Aphlonobia histricina, Eutetranychus banksi, E. orientalis, Oligonychus perseae, Panonychus citri, Tetranychus evansi, T. okinawanus and T. turkestani, and the phytoseiids Amblyseius swirskii, A. cucumeris, A. andersoni, Euseius stipulatus, Neoseiulus barkeri, N. californicus, Phytoseiulus persimilis and Typhlodromus phialatus. Eight successful cross amplifications were obtained.ca_CA
dc.format.extent15 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherSpringer Verlagca_CA
dc.relation.isPartOfExperimental and Applied Acarology May 2012, Volume 57, Issue 1ca_CA
dc.subjectCitrus clementinaca_CA
dc.subjectMicrosatellitesca_CA
dc.subjectSSRsca_CA
dc.subjectIPMca_CA
dc.titleIsolation and characterization of polymorphic microsatellite markers in Tetranychus urticae and cross amplification in other Tetranychidae and Phytoseiidae species of economical importanceca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1007/s10493-012-9529-x
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_CA
dc.relation.publisherVersionThe final publication is available at link.springer.com: http://dx.doi.org/10.1007/s10493-012-9529-xca_CA


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