Isolation and characterization of polymorphic microsatellite markers in Tetranychus urticae and cross amplification in other Tetranychidae and Phytoseiidae species of economical importance
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Other documents of the author: Sabater-Muñoz, Beatriz; Pascual Ruiz, Sara; Gómez-Martínez, María Antonia; Jaques , Josep A.; Hurtado-Ruiz, Monica
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comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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Title
Isolation and characterization of polymorphic microsatellite markers in Tetranychus urticae and cross amplification in other Tetranychidae and Phytoseiidae species of economical importanceAuthor (s)
Date
2012Publisher
Springer VerlagISSN
0168-8162; 1572-9702Bibliographic citation
Sabater-Muñoz, B., Pascual-Ruiz, S., Gómez-Martínez, M. A., Jacas, J. A., & Hurtado, M. A. (2012). Isolation and characterization of polymorphic microsatellite markers in Tetranychus urticae and cross amplification in other Tetranychidae and Phytoseiidae species of economic importance. Experimental and Applied Acarology, 57(1), 37-51.Type
info:eu-repo/semantics/articlePublisher version
The final publication is available at link.springer.com: http://dx.doi.org/10.1007/s10493-012-9529-xSubject
Abstract
Tetranychus urticae Koch is a cosmopolitan phytophagous mite considered as
the most polyphagous species among spider mites. Population genetic studies using
molecular markers such as microsatellites have proven to ... [+]
Tetranychus urticae Koch is a cosmopolitan phytophagous mite considered as
the most polyphagous species among spider mites. Population genetic studies using
molecular markers such as microsatellites have proven to be extremely informative to
address questions about population structure, phylogeography and host preferences. The
aim of this study was to increase the available molecular tools to gain insight into the
genetic structure of T. urticae populations of citrus orchards, which might help in their
management. Five microsatellite DNA libraries were developed using probes with the
motifs CT, CTT, GT and CAC following the FIASCO protocol. Positive clones, those that
included the insert with the microsatellite, were detected using the PIMA-PCR technique.
Combinations of primers were designed on 22 out of 32 new microsatellites loci and their
polymorphism was tested in four populations sampled along the eastern coast of Spain.
Eleven successful amplifications were obtained. Cross amplification was tested in the
tetranychids Aphlonobia histricina, Eutetranychus banksi, E. orientalis, Oligonychus
perseae, Panonychus citri, Tetranychus evansi, T. okinawanus and T. turkestani, and the
phytoseiids Amblyseius swirskii, A. cucumeris, A. andersoni, Euseius stipulatus, Neoseiulus
barkeri, N. californicus, Phytoseiulus persimilis and Typhlodromus phialatus. Eight
successful cross amplifications were obtained. [-]
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Experimental and Applied Acarology May 2012, Volume 57, Issue 1Rights
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