Monitoring disopyramide, lidocaine and quinidine by micellar liquid chromatography.
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Otros documentos de la autoría: Esteve-Romero, Josep; Rambla Alegre, María; Ochoa Aranda, Enrique; Martinavarro Domínguez, Adrià; Marcos Tomás, José V.; Bose, Devasish
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Título
Monitoring disopyramide, lidocaine and quinidine by micellar liquid chromatography.Autoría
Fecha de publicación
2011-04Editor
© 2011 AOAC INTERNATIONALISSN
1060-3271Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://aoac.publisher.ingentaconnect.com/content/aoac/jaoac/2011/00000094/000000 ...Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
A micellar liquid chromatography (MLC) method using a C18 column was developed to determine three antiarrhythmic drugsdisopyramide, lidocaine, and quinidinethat are most usually monitored in serum samples. After the ... [+]
A micellar liquid chromatography (MLC) method using a C18 column was developed to determine three antiarrhythmic drugsdisopyramide, lidocaine, and quinidinethat are most usually monitored in serum samples. After the application of an interpretative strategy for optimization of sodium dodecyl sulfate (SDS) and modifier concentrations in order to ensure the minimum analysis time, maximum sensitivity, and good resolution, the optimum chromatographic conditions for the determination of the three antiarrhythmics were flow rate, 1 mL/min; injection volume, 20 L; separation temperature, 25C; mobile phase, 150 mmol/L SDS-7 (v/v) butanolphosphate buffer, 10 mmol/L, pH 70.9 (w/v) NaCl; and detection at 214 nm. The calibration curves for the drugs were linear (r2 > 0.999). The intraday and interday precisions were lower than 3.9 (CV). Recoveries were 100 0.6 when the method was applied to both serum samples spiked with the antiarrhythmics (n 10) and real serum samples. In all cases, the results were similar to those obtained using the reference method (fluorescence polarization immunoassay) usually used in the Spanish hospital. The proposed method is useful for hospital monitoring of the antiarrhythmics by direct injection into the chromatograph. [-]
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Journal of AOAC International (March-April 2011 ), vol. 94, no. 2, 537-542Derechos de acceso
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