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Linearity, saturation and blocking in a large multiionic channel: divalent cation modulation of the OmpF porin conductance
dc.contributor.author | García Giménez, Elena | |
dc.contributor.author | López Peris, María Lidón | |
dc.contributor.author | Aguilella, Vicente | |
dc.contributor.author | Alcaraz, Antonio | |
dc.date.accessioned | 2012-09-04T11:48:48Z | |
dc.date.available | 2012-09-04T11:48:48Z | |
dc.date.issued | 2011-01 | |
dc.identifier.citation | Biochemical and Biophysical Research Communications (7 Jan. 2011), vol. 404, no. 1, 330–334 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.issn | 1090-2104 | |
dc.identifier.uri | http://hdl.handle.net/10234/45911 | |
dc.description.abstract | Measurement of unitary conductance is a fundamental step in the characterization of a protein ion channelpermeabilizing a membrane. We study here the effect of salts of divalent cations on the OmpF channelconductance with a particular emphasis in dissecting the role of the electrolyte itself, the role of the counterion accumulation induced by the protein channel charges and other effects not found in salts of monovalent cations. We show that current saturation and blocking are not exclusive properties of narrow (single-file) ion channels but may be observed in large, multiionic channels like bacterial porins. Single-channel conductance measurements performed over a wide range of salt concentrations (up to 3 M) combined with continuum electrodiffusion calculations demonstrate that current saturation cannot be simply ascribed to ion interaction with protein channel residues. | ca_CA |
dc.description.sponsorShip | Support from the Spanish Ministry of Science and Innovation, (MICINN Projects No. FIS2007-60205 and FIS2010-19810) and Fundació Caixa Castelló-Bancaixa (Project No. P1-1A2009-13) | ca_CA |
dc.format.extent | 5 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language | eng | ca_CA |
dc.language.iso | cat | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.rights | © 2010 Elsevier Inc. All rights reserved | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Ion channel | ca_CA |
dc.subject | Electrical conductance | ca_CA |
dc.subject | Bacterial porin | ca_CA |
dc.subject | Divalent cations | ca_CA |
dc.title | Linearity, saturation and blocking in a large multiionic channel: divalent cation modulation of the OmpF porin conductance | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1016/j.bbrc.2010.11.118 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0006291X10021935 | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion |
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