Tamoxifen monitoring studies in breast cancer patients by micellar liquid chromatography
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Otros documentos de la autoría: Esteve-Romero, Josep; Ochoa Aranda, Enrique; Bose, Devasish; Rambla Alegre, María; Vives-Peris, Vicente; Martinavarro Domínguez, Adrià
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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Título
Tamoxifen monitoring studies in breast cancer patients by micellar liquid chromatographyAutoría
Fecha de publicación
2010Editor
Springer VerlagISSN
1618-2642Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://link.springer.com/article/10.1007%2Fs00216-010-3695-7Versión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
A simple micellar liquid chromatographic procedure is described to determine tamoxifen in plasma. To perform the analysis, tamoxifen solutions were diluted in water and UV- irradiated for 20 min to form the photocycled ... [+]
A simple micellar liquid chromatographic procedure is described to determine tamoxifen in plasma. To perform the analysis, tamoxifen solutions were diluted in water and UV- irradiated for 20 min to form the photocycled derivative with a phenanthrene core which shows intense fluorescence. Samples were then directly injected, thus avoiding long extraction and experimental procedures. The resolution from the matrix was performed with a mobile phase containing 0.15 M SDS – 7% n-butanol at pH 3 running at 1.5 ml/min through a C18 column at 40oC. Detection was carried out by fluorescence, and the excitation and emission wavelengths were 260 and 380 nm, respectively. The chromatographic analysis time was less than 15 min. The analytical methodology was validated following the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The response of the drug in plasma was linear and in the 0.5-15 μg/ml range, with r2 > 0.999. Accuracy and precision were < 9% in both cases. The limits of detection and quantification (ng/ml) were 50 and 150 in plasma, respectively. The method developed herein shows no interferences by endogenous compounds. Finally, the analytical method was used to determine the amount of tamoxifen in the plasma of several breast cancer patients from a local hospital. [-]
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Analytical and Bioanalytical Chemistry, Vol. 397, No. 4 (2010), p. 1557-1561Derechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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