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dc.contributor.authorRomero-Gavilán, Francisco J
dc.contributor.authorGomes, Nuno
dc.contributor.authorGarcía-Arnáez, Iñaki
dc.contributor.authorMartínez‑Ramos, C.
dc.contributor.authorElortza, Felix
dc.contributor.authorAzkargorta, Mikel
dc.contributor.authorIloro, Ibon
dc.contributor.authorGURRUCHAGA, MARILO
dc.contributor.authorSuay Antón, Julio José
dc.contributor.authorGoñi, Isabel
dc.date.accessioned2019-05-09T11:08:11Z
dc.date.available2019-05-09T11:08:11Z
dc.date.issued2019-02-01
dc.identifier.citationROMERO-GAVILÁN, F., et al. The effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactions. Colloids and Surfaces B: Biointerfaces, 2019, vol. 174, p. 9-16ca_CA
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.urihttp://hdl.handle.net/10234/182450
dc.description.abstractIt is known strontium can both inhibit the osteoclast formation and stimulate the osteoblast maturation, so biomaterials containing this element can favour bone structure stabilisation. The addition of Sr to biomaterials could affect their interactions with proteins and cells. Here, a silica-hybrid sol-gel network doped with different amounts of SrCl2 and applied as coatings on titanium discs was examined. in vitro analysis was performed to determine the potential effect of Sr in the coatings, showing enhanced gene expression of osteogenic markers (alkaline phosphatase and transforming growth factor-β) in MC3T3-E1 incubated with Sr-doped biomaterials. The examination of inflammatory markers (tumour necrosis factor-α and interleukin 10) in RAW 264.7 macrophages revealed an anti-inflammatory potential of these materials. Proteins adsorbed onto the coatings incubated with human serum (3 h at 37 °C) were also analysed; mass spectrometry was used to characterise the proteins adhering to materials with different Sr content. Adding Sr to the coatings increased their affinity to APOE and VTNC proteins (associated with anti-inflammatory and osteogenic functions). Moreover, the proteins involved in coagulation processes, such as prothrombin, were more abundant on the coatings containing Sr than on the base sol-gel surfaces. Correlations between gene expression and proteomic results were also examined.ca_CA
dc.format.extent8 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherElsevierca_CA
dc.relation.isPartOfColloids and Surfaces B: Biointerfaces, 2019, vol. 174ca_CA
dc.rightsCopyright © Elsevierca_CA
dc.subjectdental implantsca_CA
dc.subjectapolipoprotein Eca_CA
dc.subjectvitronectinca_CA
dc.subjectosteogenesisca_CA
dc.subjectbone regenerationca_CA
dc.subjectproteomicsca_CA
dc.titleThe effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactionsca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttps://doi.org/10.1016/j.colsurfb.2018.10.075
dc.relation.projectIDMINECO: MAT2017-86043-R); Universitat Jaume I: Predoc/2014/25, UJI-B2017-37); Basque Government: IT611-13, Predoc/2016/1/0141; University of the Basque Country: UFI11/56ca_CA
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_CA
dc.relation.publisherVersionhttps://www.sciencedirect.com/science/article/pii/S0927776518307707ca_CA
dc.type.versioninfo:eu-repo/semantics/submittedVersionca_CA


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