Show simple item record

dc.contributor.authorIzquierdo Sandoval, David
dc.contributor.otherIbáñez Martínez, María
dc.contributor.otherUniversitat Jaume I. Departament de Química Física i Analítica
dc.descriptionTreball Final de Grau en Química. Codi: QU0943. Curs acadèmic: 2017/2018ca_CA
dc.description.abstractThis project is based on the building of an empirical mass spectra library with the aim of confirming hormone structures in semi-quantitative non-targeted metabolomics analysis in plants. The library was composed by twenty phenolic compounds: kaempferol, apigenin, neohesperedin, ferulic acid, caffeic acid, chlorogenic acid, 3-dehidroshikimate, 4-coumaryl alcohol, capsaicin, (-) epicatechin, ampelopsin, taxifolin, gentisic acid, coumarine, quercitrin, epigallocatechin, skimmianine, catechin, sinapic acid and naringin. Ultra-high performance-liquid chromatography coupled to hybrid quadrupole time-of-flight mass spectrum (UHPLC-QTOF MS) has been used in order to acquire retention time at the same time that low and high collision energy spectra. On the other hand, the effectiveness of the extraction method used for this type of analysis in the analytes of the library is checked. For this purpose, samples of Arabidopsis thaliana and Solanum lycopersicum (tomato) spiked at several levels were analysed and their spectra compared with those of pure standards at the same levels. Finally, the library has been applied to an on-going research consisting in the metabolomic comparison between adult and seedlings plants of Arabidopsis thaliana treated with I3CA against the infection of P. cucumerina.ca_CA
dc.format.extent116 p.ca_CA
dc.publisherUniversitat Jaume Ica_CA
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectGrau en Químicaca_CA
dc.subjectGrado en Químicaca_CA
dc.subjectBachelor's Degree in Chemistryca_CA
dc.titleBuilding an empirical mass spectra libray for non-targeted metabolomics analysis in plants by UHPLC-QTOF MSca_CA
dc.educationLevelEstudios de Gradoca_CA

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional