Building an empirical mass spectra libray for non-targeted metabolomics analysis in plants by UHPLC-QTOF MS
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/158176
comunitat-uji-handle2:10234/71324
comunitat-uji-handle3:10234/98504
comunitat-uji-handle4:
TFG-TFMMetadatos
Título
Building an empirical mass spectra libray for non-targeted metabolomics analysis in plants by UHPLC-QTOF MSAutoría
Tutor/Supervisor; Universidad.Departamento
Ibáñez Martínez, María; Universitat Jaume I. Departament de Química Física i AnalíticaFecha de publicación
2018-07Editor
Universitat Jaume IResumen
This project is based on the building of an empirical mass spectra library with
the aim of confirming hormone structures in semi-quantitative non-targeted
metabolomics analysis in plants. The library was composed ... [+]
This project is based on the building of an empirical mass spectra library with
the aim of confirming hormone structures in semi-quantitative non-targeted
metabolomics analysis in plants. The library was composed by twenty phenolic
compounds: kaempferol, apigenin, neohesperedin, ferulic acid, caffeic acid,
chlorogenic acid, 3-dehidroshikimate, 4-coumaryl alcohol, capsaicin, (-)
epicatechin, ampelopsin, taxifolin, gentisic acid, coumarine, quercitrin,
epigallocatechin, skimmianine, catechin, sinapic acid and naringin. Ultra-high
performance-liquid chromatography coupled to hybrid quadrupole time-of-flight
mass spectrum (UHPLC-QTOF MS) has been used in order to acquire retention
time at the same time that low and high collision energy spectra.
On the other hand, the effectiveness of the extraction method used for this type
of analysis in the analytes of the library is checked. For this purpose, samples of
Arabidopsis thaliana and Solanum lycopersicum (tomato) spiked at several levels
were analysed and their spectra compared with those of pure standards at the same
levels.
Finally, the library has been applied to an on-going research consisting in the
metabolomic comparison between adult and seedlings plants of Arabidopsis
thaliana treated with I3CA against the infection of P. cucumerina. [-]
Palabras clave / Materias
Descripción
Treball Final de Grau en Química. Codi: QU0943. Curs acadèmic: 2017/2018
Tipo de documento
info:eu-repo/semantics/bachelorThesisDerechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- Grau en Química [265]
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