Epigenetic regulation of the expression of WRKY75 transcription factor in response to biotic and abiotic stresses in Solanaceae plants
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Other documents of the author: López-Galiano, María José; González-Hernández, Ana I.; Crespo Salvador, Óscar; Rausell, Carolina; Real, María Dolores; Escamilla, Mónica; Camañes, Gemma; García Agustín, Pilar; González-Bosch, Carmen; García-Robles, Inmaculada
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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https://doi.org/10.1007/s00299-017-2219-8 |
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Title
Epigenetic regulation of the expression of WRKY75 transcription factor in response to biotic and abiotic stresses in Solanaceae plantsAuthor (s)
Date
2017Publisher
Springer VerlagISSN
0721-7714; 1432-203XBibliographic citation
López-Galiano, M.J., González-Hernández, A.I., Crespo-Salvador, O. et al. Plant Cell Rep (2018) 37: 167. https://doi.org/10.1007/s00299-017-2219-8Type
info:eu-repo/semantics/articlePublisher version
https://link.springer.com/article/10.1007%2Fs00299-017-2219-8#citeasVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Key message
SlyWRKY75: gene expression was induced in response to biotic stresses, especially in Botrytis cinerea-infected tomato plants, in which Sly-miR1127-3p is a putative SlyWRKY75 regulator and epigenetic marks ... [+]
Key message
SlyWRKY75: gene expression was induced in response to biotic stresses, especially in Botrytis cinerea-infected tomato plants, in which Sly-miR1127-3p is a putative SlyWRKY75 regulator and epigenetic marks were detected.
Abstract
WRKY75 transcription factor involved in Pi homeostasis was recently found also induced in defense against necrotrophic pathogens. In this study, we analyzed by RT-qPCR the expression of SlyWRKY75 gene in tomato plants in response to abiotic stresses (drought or heat) and biotic stresses (Colorado potato beetle larvae infestation, Pseudomonas syringae or Botrytis cinerea infection) being only differentially expressed following biotic stresses, especially upon B. cinerea infection (55-fold induction). JA and JA-Ile levels were significantly increased in tomato plants under biotic stresses compared with control plants, indicating that SlyWRKY75 might be a transcriptional regulator of the JA pathway. The contribution of miRNAs and epigenetic molecular mechanisms to the regulation of this gene in B. cinerea-infected tomato plants was explored. We identified a putative Sly-miR1127-3p miRNA predicted to bind the intronic region of the SlyWRKY75 genomic sequence. Sly-miR1127-3p miRNA was repressed in infected plants (0.4-fold) supporting that it might act as an epigenetic regulation factor of SlyWRKY75 gene expression rather than via the post-transcriptional mechanisms of canonical miRNAs. It has been proposed that certain miRNAs can mediate DNA methylation in the plant nucleus broadening miRNA functions with transcriptional gene silencing by targeting intron-containing pre-mRNAs. Histone modifications analysis by chromatin immunoprecipitation (ChIP) demonstrated the presence of the activator histone modification H3K4me3 on SlyWRKY75 transcription start site and gene body. The induction of this gene in response to B. cinerea correlates with the presence of an activator mark. Thus, miRNAs and chromatin modifications might cooperate as epigenetic factors to modulate SlyWRKY75 gene expression. [-]
Is part of
Plant Cell Rep (2018) 37Investigation project
AGL2013-49023-C3-1,2,3-R ; PREDOC/2016/27 ; AGL2013-49023-C03-01Rights
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
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