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dc.contributor.authorKalu, Nnanya
dc.contributor.authorAlcaraz, Antonio
dc.contributor.authorYamini, Goli
dc.contributor.authorMombem Abolfath, Sanaz
dc.contributor.authorLucas, Laura
dc.contributor.authorKenney, Clare
dc.contributor.authorAguilella, Vicente
dc.contributor.authorNestorovich, Ekaterina M.
dc.date.accessioned2018-02-15T12:02:41Z
dc.date.available2018-02-15T12:02:41Z
dc.date.issued2017-11
dc.identifier.citationKALU, Nnanya; ALCARAZ GONZÁLEZ, Antonio; YAMINI, Goli; MOMBEM ABOLFATH, Sanaz; LUCAS, Laura; KENNEY, Clare; AGUILELLA FERNÁNDEZ, Vicente; NESTOROVICH, Ekaterina M. Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel. FEBS Letters (2017), v. 591, issue 21, p. 3481-3492ca_CA
dc.identifier.urihttp://hdl.handle.net/10234/172850
dc.description.abstractTight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ by many intracellularly active bacterial exotoxins, including the anthrax toxin. Here we investigate the role of pH on single-channel properties of the anthrax toxin channel, PA63. Using conductance and current noise analysis, blocker binding, ion selectivity, and PEG partitioning measurements, we show that the channel exists in two different open states (‘maximum’ and ‘main’) at pH  5.5, while only a maximum conductance state is detected at pH  5.5. We describe two substantially distinct patterns of PA63 conductance dependence on KCl concentration uncovered at pH 6.5 and 4.5.ca_CA
dc.format.extent20 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherWileyca_CA
dc.relation.isPartOfFEBS Letters (2017), v. 591, issue 21, p. 3481-3492ca_CA
dc.rights.urihttp://rightsstatements.org/vocab/CNE/1.0/*
dc.subjectAccess resistanceca_CA
dc.subjectBacillus anthracisca_CA
dc.subjectCurrent fluctuationsca_CA
dc.subjectEndosomal acidificationca_CA
dc.subjectMulti-state conductanceca_CA
dc.titleEffect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channelca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1002/1873-3468.12866
dc.relation.projectIDThe project was financially supported by: 1) NIAID of the NIH under award number 1R15AI099897-01A1; 2) The Catholic University startup funds (to Ekaterina Nestorovich); 3) Spanish Government (FIS2013-40473-P and FIS2016-75257-P AEI/FEDER), 4) Universitat Jaume I (P1.1B2015-28); 5) American Dissertation Fellowship from AAUWca_CA
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_CA
dc.relation.publisherVersionhttp://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12866/abstract;jsessionid=6BAD47FFD1134A703A5765F4617E8F52.f04t01ca_CA
dc.type.versioninfo:eu-repo/semantics/submittedVersionca_CA


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