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Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel
dc.contributor.author | Kalu, Nnanya | |
dc.contributor.author | Alcaraz, Antonio | |
dc.contributor.author | Yamini, Goli | |
dc.contributor.author | Mombem Abolfath, Sanaz | |
dc.contributor.author | Lucas, Laura | |
dc.contributor.author | Kenney, Clare | |
dc.contributor.author | Aguilella, Vicente | |
dc.contributor.author | Nestorovich, Ekaterina M. | |
dc.date.accessioned | 2018-02-15T12:02:41Z | |
dc.date.available | 2018-02-15T12:02:41Z | |
dc.date.issued | 2017-11 | |
dc.identifier.citation | KALU, Nnanya; ALCARAZ GONZÁLEZ, Antonio; YAMINI, Goli; MOMBEM ABOLFATH, Sanaz; LUCAS, Laura; KENNEY, Clare; AGUILELLA FERNÁNDEZ, Vicente; NESTOROVICH, Ekaterina M. Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel. FEBS Letters (2017), v. 591, issue 21, p. 3481-3492 | ca_CA |
dc.identifier.uri | http://hdl.handle.net/10234/172850 | |
dc.description.abstract | Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ by many intracellularly active bacterial exotoxins, including the anthrax toxin. Here we investigate the role of pH on single-channel properties of the anthrax toxin channel, PA63. Using conductance and current noise analysis, blocker binding, ion selectivity, and PEG partitioning measurements, we show that the channel exists in two different open states (‘maximum’ and ‘main’) at pH 5.5, while only a maximum conductance state is detected at pH 5.5. We describe two substantially distinct patterns of PA63 conductance dependence on KCl concentration uncovered at pH 6.5 and 4.5. | ca_CA |
dc.format.extent | 20 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Wiley | ca_CA |
dc.relation.isPartOf | FEBS Letters (2017), v. 591, issue 21, p. 3481-3492 | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/CNE/1.0/ | * |
dc.subject | Access resistance | ca_CA |
dc.subject | Bacillus anthracis | ca_CA |
dc.subject | Current fluctuations | ca_CA |
dc.subject | Endosomal acidification | ca_CA |
dc.subject | Multi-state conductance | ca_CA |
dc.title | Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1002/1873-3468.12866 | |
dc.relation.projectID | The project was financially supported by: 1) NIAID of the NIH under award number 1R15AI099897-01A1; 2) The Catholic University startup funds (to Ekaterina Nestorovich); 3) Spanish Government (FIS2013-40473-P and FIS2016-75257-P AEI/FEDER), 4) Universitat Jaume I (P1.1B2015-28); 5) American Dissertation Fellowship from AAUW | ca_CA |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12866/abstract;jsessionid=6BAD47FFD1134A703A5765F4617E8F52.f04t01 | ca_CA |
dc.type.version | info:eu-repo/semantics/submittedVersion | ca_CA |
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