Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus
Scholar | Other documents of the author: Merelo, Paz; Agustí, Javier; Arbona, Vicent; Costa, Mario L.; Estornell, Leandro H.; Gómez Cadenas, Aurelio; Coimbra, Silvia; Gómez, María D.; Pérez Amador, Miguel A.; Domingo, Concha; Talón, Manuel; Tadeo, Francisco R.
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TitleCell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus
Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The process takes place in specific locations termed abscission zones. In fruit crops like citrus, fruit ... [+]
Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The process takes place in specific locations termed abscission zones. In fruit crops like citrus, fruit abscission represents a high percentage of annual yield losses. Thus, understanding the molecular regulation of abscission is of capital relevance to control production. To identify genes preferentially expressed within the citrus fruit abscission zone (AZ-C), we performed a comparative transcriptomics assay at the cell type resolution level between the AZ-C and adjacent fruit rind cells (non-abscising tissue) during ethylene-promoted abscission. Our strategy combined laser microdissection with microarray analysis. Cell wall modification-related gene families displayed prominent representation in the AZ-C. Phylogenetic analyses of such gene families revealed a link between phylogenetic proximity and expression pattern during abscission suggesting highly conserved roles for specific members of these families in abscission. Our transcriptomic data was validated with (and strongly supported by) a parallel approach consisting on anatomical, histochemical and biochemical analyses on the AZ-C during fruit abscission. Our work identifies genes potentially involved in organ abscission and provides relevant data for future biotechnology approaches aimed at controlling such crucial process for citrus yield. [-]
Investigation project1) Spanish Instituto Nacional de Investigaciones Agrarias (grant RTA2008-00065-00-00 to FT [including a PhD fellowship for PM] and RTA2014-00071-C06-01 to MT); 2) Spanish Ministerio de Economia e Innovación (grants PSE- 060000-2009-8 and IPT-010000-2010-43 to MT and BIO2011- 26302 to MP); 3) Spanish Ministerio de Industria (grant AGL2011-30240 to MT); 4) “Juan de la Cierva” and an INIA/CCAA postdoctoral contract, respectively.
Bibliographic citationMERELO, Paz; AGUSTÍ, Javier; ARBONA MENGUAL, Vicent; COSTA, Mario L.; ESTORNELL, Leandro H.; GÓMEZ CADENAS, Aurelio; COIMBRA, Silvia; GÓMEZ, María D.; PÉREZ AMADOR, Miguel A.; DOMINGO, Concha; TALÓN, Manuel; TADEO, Francisco R. Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus. Frontiers in Plant Science (2017), v. 8
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