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dc.contributor.authorFernández Crespo, Emma
dc.contributor.authorLópez Cruz, J.
dc.contributor.authorCrespo Salvador, Óscar
dc.contributor.authorGarcía Agustín, Pilar
dc.contributor.authorGonzález Bosch, Carmen
dc.identifier.citationLópez-Cruz, J., Óscar, C.-S., Emma, F.-C., Pilar, G.-A. and Carmen, G.-B. (2017), Absence of Cu–Zn superoxide dismutase BCSOD1 reduces Botrytis cinerea virulence in Arabidopsis and tomato plants, revealing interplay among reactive oxygen species, callose and signalling pathways. Molecular Plant Pathology, 18: 16–31. doi:10.1111/mpp.12370
dc.description.abstractPlants activate responses against pathogens, including the oxida-tive burst. Necrotrophic pathogens can produce reactive oxygenspecies (ROS) that benefit the colonization process. Previously, wehave demonstrated that tomato plants challenged with Botrytiscinerea accumulate ROS and callose, together with the inductionof genes involved in defence, signalling and oxidative metabolism.Here, we studied the infection phenotype of the Dbcs od1 strain inboth tomato and Arabidopsis plants. This mutant lacks bcsod1,which encodes Cu–Zn superoxide dismutase (SOD). This enzymecatalyses the conversion of superoxide ion (O–2) into hydrogen per-oxide (H2O2). ROS play a protective role and act as signals inplants. Dbcsod1 displayed reduced virulence compared with wild-type B05.10 in both species. Plants infected with Dbcsod1 accu-mulated less H2O2and more O–2than those infected with B05.10,which is associated with an increase in the defensive polymer cal-lose. This supports a major role of fungal SOD in H2O2productionduring the plant–pathogen interaction. The early induction of thecallose synthase gene PMR4 suggested that changes in ROSaltered plant defensive responses at the transcriptional level. Themetabolites and genes involved in signalling and in response tooxidative stress were differentially expressed on Dbcsod1 infec-tion, supporting the notion that plants perceive changes in ROSbalance and activate defence responses. A higher O2–/H2O2ratioseems to be beneficial for plant protection against this necrotroph.Our results highlight the relevance of callose and the oxylipin 12-oxo-phytodienoic acid (OPDA) in the response to changes in theoxidative environment, and clarify the mechanisms that underliethe responses to Botrytis in Arabidopsis and tomato plants.ca_CA
dc.description.sponsorShipThis work was supported by grants from the Spanish Ministry of Scienceand Innovation (AGL2010-22300-C03-01-02 and AGL2013-49023-C03-01-02), co-funded by the European Regional Development Funds (ERDF)and by Generalitat Valenciana Grupos de Excelencia PROMETEO/2012/066. J.L.-C. and O.C.-S. were recipients of research contracts from thePROMETEO/2012/066 grant. E.F.-C. was the recipient of research con-tracts from grant AGL2010-22300-C03-01. J.L.-C. is the recipient of aresearch contract from grant AGL2013-49023-C03-01.ca_CA
dc.format.extent16 p.ca_CA
dc.relation.isPartOfMolecular Plant Pathology (2017) 18(1), 16–31ca_CA
dc.rights© 2016 BSPP AND JOHN WILEY & SONS LTDca_CA
dc.subjectArabidopsis thalianaca_CA
dc.subjectBotrytis cinereaca_CA
dc.subjectSolanum lycopersicumca_CA
dc.titleAbsence of Cu–Zn superoxide dismutase BCSOD1 reduces Botrytiscinerea virulence in Arabidopsis and tomato plants, revealinginterplay among reactive oxygen species, callose and signallingpathwaysca_CA

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