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dc.contributor.authorPerez-Pouchoulen, Miguel
dc.contributor.authorMiquel, Marta
dc.contributor.authorSaft, Paul
dc.contributor.authorBrug, Brenda
dc.contributor.authorToledo, Rebeca
dc.contributor.authorHernández, María Elena
dc.contributor.authorManzo, Jorge
dc.date.accessioned2016-09-02T09:55:36Z
dc.date.available2016-09-02T09:55:36Z
dc.date.issued2016-10
dc.identifier.citationPEREZ-POUCHOULEN, Miguel, et al. Prenatal exposure to sodium valproate alters androgen receptor expression in the developing cerebellum in a region and age specific manner in male and female rats. International Journal of Developmental Neuroscience, 2016, vol. 53, p. 46-52ca_CA
dc.identifier.issn0736-5748
dc.identifier.urihttp://hdl.handle.net/10234/162172
dc.description.abstractValproic acid (VPA) is an anti-epileptic drug with teratogenicity activity that has been related to autism. In rodents, exposure to VPA in utero leads to brain abnormalities similar than those reported in the autistic brain. Particularly, VPA reduces the number of Purkinje neurons in the rat cerebellum parallel to cerebellar abnormalities found in autism. Thus, we injected pregnant females on embryonic day 12 either with VPA (600 mg/kg, i.p.) or 0.9% saline solution and obtained the cerebellum from their offspring at different postnatal time points. Testosterone has been linked to autism and plays an important role during brain development. Therefore, we identified and analyzed the androgen receptor (AR) by immunohistochemistry and densitometry, respectively. We found VPA decreases AR density in the superficial Purkinje layer only in cerebellar lobule 8 at PN7, but increased it at PN14 compared to control in males. In females, VPA decreased AR density in the superficial Purkinje layer in cerebellar lobule 6 at PN14, but increased it in lobule 9 at the same time point. No differences were found in the deep Purkinje layer of any cerebellar lobule in terms of AR density neither in males nor females. We additionally found a particular AR density decreasing in both superficial and deep regions across development in the majority of cerebellar lobules in males, but in all cerebellar lobules in females. Thus, our results indicate that VPA disrupts the AR ontogeny in the developing cerebellum in an age and region specific manner in male and female rats. Future epigenetic studies including the evaluation of histone deacetylases (HDAC’s) might shed light these results as HDAC’s are expressed by Purkinje neurons, interact with the AR and are VPA targets. This work contributes to the understanding of the cerebellar development and it might help to understand the role of the cerebellum in neurodevelopmental disorders such as autism.ca_CA
dc.description.sponsorShipThis research was supported by CONACYT (Consejo Nacional de Ciencia y Tecnologia of Mexico Grant 106531 to Maria Elena Hernandez (MEH) and CONACYT Doctorate scholarship 205779 to Miguel Perez Pouchoulen (MPP). Authors thank M.S. Dulce Mariely Alvarez-Croda for her valuable comments to the manuscript.ca_CA
dc.format.extent7 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherElsevierca_CA
dc.relation.isPartOfInternational Journal of Developmental Neuroscience, 2016, vol. 53ca_CA
dc.rights© 2016 ISDN. Published by Elsevier Ltd. All rights reserved.ca_CA
dc.subjectVermisca_CA
dc.subjectTeratogenca_CA
dc.subjectPurkinje neuronca_CA
dc.subjectNeurodevelopmental disordersca_CA
dc.subjectSex differencesca_CA
dc.titlePrenatal exposure to sodium valproate alters androgen receptor expression in the developing cerebellum in a region and age specific manner in male and female ratsca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1016/j.ijdevneu.2016.07.001
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_CA
dc.relation.publisherVersionhttp://www.sciencedirect.com/science/article/pii/S0736574816301174ca_CA


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