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dc.contributor.authorPeris Vicente, Juan
dc.contributor.authorOchoa Aranda, Enrique
dc.contributor.authorBose, Devasish
dc.contributor.authorEsteve-Romero, Josep
dc.date.accessioned2016-03-16T13:16:01Z
dc.date.available2016-03-16T13:16:01Z
dc.date.issued2015-01
dc.identifier.citationPERIS-VICENTE, Juan, et al. Determination of tamoxifen and its main metabolites in plasma samples from breast cancer patients by micellar liquid chromatography. Talanta, 2015, vol. 131, p. 535-540.ca_CA
dc.identifier.urihttp://hdl.handle.net/10234/154068
dc.description.abstractA method was developed for the analysis of tamoxifen and its main derivatives (4-hydroxytamoxifen, N–desmethyl-tamoxifen, tamoxifen-N-oxide and endoxifen) in human plasma, using micellar liquid chromatography coupled with fluorescence detection. Analytes were off-line derivatized by sample UV-irradiation for 20 min to form the photocycled fluorescent derivatives. Then samples were diluted, filtered and directly injected, thus avoiding extraction steps. The analytes were resolved using a mobile phase containing 0.08 M SDS-4.5% butanol at pH 3 running at 1.5 mL/min through a C18 column at 40 °C, without interferences from endogenous compounds in plasma. Excitation and emission wavelengths were 260 and 380 nm, respectively. The chromatographic analysis time was less than 40 min. The analytical methodology was validated following the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines in terms of: selectivity, linear range (0.3–15 μg/mL), linearity (r2>0.999), sensitivity (LOD, 65–80 ng/mL; LOQ, 165–200 ng/mL), intra- and interday accuracy (−12.2–11.5%) and precision (<9.2%) and robustness (<6.3%). The method was used to quantify the tamoxifen and tamoxifen derivatives in several breast cancer patients from a local hospital, in order to study the correlation between the genotype of the patient and the ability to metabolize tamoxifen.ca_CA
dc.format.extent5 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isospaca_CA
dc.publisherElsevierca_CA
dc.relation.isPartOfTalanta Volume 131, January 2015ca_CA
dc.rightsCopyright © 2014 Elsevier B.V. All rights reserved.ca_CA
dc.subjectGenotypeca_CA
dc.subjectMetabolizationca_CA
dc.subjectMicellar liquid chromatographyca_CA
dc.subjectPlasmaca_CA
dc.subjectTamoxifenca_CA
dc.titleDetermination of Tamoxifen and its main metabolites in plasma samples from breast cancer patients by micellar liquid chromatographyca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_CA
dc.relation.publisherVersionhttp://www.sciencedirect.com/science/article/pii/S0039914014006614ca_CA


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