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dc.contributorSerrano Gallego, Roque
dc.contributorUniversitat Jaume I. Departament de Química Física i Analítica
dc.contributor.authorGarlito Molina, Borja
dc.date.accessioned2015-11-13T09:48:34Z
dc.date.available2015-11-13T09:48:34Z
dc.date.issued2015-06-30
dc.identifier.urihttp://hdl.handle.net/10234/139985
dc.descriptionTreball Final del Màster Universitari en Tècniques Cromatogràfiques Aplicades (Pla de 2013). Codi: SIY009. Curs acadèmic 2014-2015ca_CA
dc.description.abstractLong-chain and very long-chain polyunsaturated fatty acids (LC-PUFAs, C12–22; VLCPUFAs, C24–38), play an important role in retinal, brain and reproductive functions. Deficiencies in retinal LC-PUFAs and VLC-PUFAs, as well as mutations in the enzyme responsible for elongation of very long-chain fatty acids (ELOVL4), have been associated with retinal diseases. Gas chromatography coupled to mass spectrometry (GC-MS) with election ionization has been widely applied to study VLC-PUFA. The extensive fragmentation of this source is the main drawback for further tandem MS (MS/MS) experiments. In the present work, an atmospheric pressure chemical ionization (APCI) source coupled to a quadrupole time-of-flight analyzer has been tested to study the ionization and mass spectrometric behavior of ten fatty acid methyl esters (FAMEs). The protonated molecule ([M+H]+ ) became the base peak of the spectrum for all the studied compounds, promoted, furthermore, by the addition of a 1% formic acid solution in the source. The preservation of the molecule provides more sensitive and selective precursor ions in selected reaction monitoring (SRM) based methods. In addition, several specific fragment ions have been found for each FAME group. The application of GC-APCI- (Q)TOF to the screening analysis of gilthead sea bream retina extracts made possible the detection of several suspect VLC-PUFA. The reliable identification of the compounds was supported by accurate mass measurements, the isotopic pattern of 13C and the presence of two representative m/z ions in the spectrum.ca_CA
dc.format.extent28 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherUniversitat Jaume Ica_CA
dc.subjectMàster Universitari en Tècniques Cromatogràfiques Aplicadesca_CA
dc.subjectMáster Universitario en Técnicas Cromatográficas Aplicadasca_CA
dc.subjectUniversity Master's Degree in Applied Chromatographic Techniquesca_CA
dc.subjectVLC-PUFAca_CA
dc.subject(Q)TOF MSca_CA
dc.subjectAPCIca_CA
dc.titleDetection and confirmation of very long-chain polyunsaturated fatty acids in gilthead sea bream retina extracts using gas chromatography with atmospheric pressure chemical ionization sourceca_CA
dc.typeinfo:eu-repo/semantics/masterThesisca_CA
dc.educationLevelEstudios de Postgradoca_CA
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_CA


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