Development and validation of a micellar liquid chromatography-based method to quantify melamine in swine kidney
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Scholar |
Otros documentos de la autoría: Beltrán Martinavarro, Beatriz; Vives-Peris, Vicente; Carda-Broch, Samuel; Esteve-Romero, Josep
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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http://dx.doi.org/10.1016/j.foodcont.2014.05.027 |
Metadatos
Título
Development and validation of a micellar liquid chromatography-based method to quantify melamine in swine kidneyAutoría
Fecha de publicación
2014Editor
ElsevierISSN
0956-7135Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.sciencedirect.com/science/article/pii/S0956713514002734Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Melamine is a toxic compound illegally added to animal feed to falsely boost protein content. This represents a strong threat to consumer's health, as melamine can reach human through the food chain. An easy and ... [+]
Melamine is a toxic compound illegally added to animal feed to falsely boost protein content. This represents a strong threat to consumer's health, as melamine can reach human through the food chain. An easy and reliable micellar liquid chromatography-method was developed to detect melamine in swine kidney. The analyte was extracted by shaking in methanol. Melamine was eluted from the HPLC column without interferences in <8.0 min. Mobile phase was a 0.11 M sodium dodecyl sulfate – 7.5% propanol at pH 3 solution running under isocratic mode through a C18 column at 1 mL/min and absorbance detection at 210 nm. The method was validated according to the Food and Drug Administration guidelines: sensitivity, calibration range (0.3–30 μg/g), linearity (r2 > 0.9997), precision (<7.6%), accuracy (−8.3–3.6%), recovery (82.1–92.4%) and robustness (<5.1%). The methodology was applied to swine kidney samples purchased from a local supermarket. The method can be used as a screening method for routine post-mortem diagnose melamine intoxication in animals and detect feeding with poultry containing melamine, in order to withdraw the corresponding flesh. This would be mandatory to prevent consumer intoxication and improve the quality of food. [-]
Publicado en
Food Control, 2014, vol. 46, p. 168-173.Derechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
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