Determination of 17b-estradiol and 17a-ethinylestradiol in water at sub-ppt levels by liquid chromatography coupled to tandem mass spectrometry
Impacto
Scholar |
Otros documentos de la autoría: Ripollés Vidal, Cristina; Ibáñez, Maria; Sancho, Juan V; Lopez, Francisco; Hernandez, Felix
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/33596
comunitat-uji-handle3:10234/33597
comunitat-uji-handle4:
INVESTIGACIONEste recurso está restringido
http://dx.doi.org/10.1039/c4ay00479e |
Metadatos
Título
Determination of 17b-estradiol and 17a-ethinylestradiol in water at sub-ppt levels by liquid chromatography coupled to tandem mass spectrometryFecha de publicación
2014-06Editor
Royal Society of ChemistryCita bibliográfica
RIPOLLÉS VIDAL, C. Determination of 17b-estradiol and 17a-ethinylestradiol in water at sub-ppt levels by liquid chromatography coupled to tandem mass spectrometry. Analytical Methods, v. 6 (June 2014), pp. 5028-5037Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://pubs.rsc.org/en/content/articlepdf/2014/ay/c4ay00479ePalabras clave / Materias
Resumen
Steroid estrogens are a group of endocrine disrupting compounds (EDCs) excreted in human urine mainly as glucuronide or sulfate conjugates, which might be converted back into the free estrogens in water. The natural ... [+]
Steroid estrogens are a group of endocrine disrupting compounds (EDCs) excreted in human urine mainly as glucuronide or sulfate conjugates, which might be converted back into the free estrogens in water. The natural estrogen 17b-estradiol (E2) and the synthetic one 17a-ethinylestradiol (EE2) are of particular concern due to their strong estrogenic activity. The main environmental source of estrogen exposure is treated
wastewaters, with the associated risk of releasing these pollutants to surface waters and even reaching finished drinking waters. In this work, we have developed and validated (at 0.1 and 1 ng L 1 levels) a robust and sensitive method based on Ultra-High Performance Liquid Chromatography (UHPLC) coupled to MS/MS for the reliable identi
fication and quantification of E2 and EE2 in surface waters.
Several sulfate conjugates have been also included into the methodology. A re-concentration step based on off-line solid-phase extraction with Oasis HLB cartridges has been optimized prior to the UHPLC-(ESI)MS/MS determination with a last-generation triple quadrupole analyzer. Two isotopelabelled analytes (E2-d4 and EE2-d4) have been used as surrogate standards to ensure an accurate quantification, achieving very low limits of detection for E2 (0.06 ng L 1 ) and EE2 (0.02 ng L 1 ). Several surface waters and influents and effluents from wastewater treatment plants have been analyzed to test the method applicability. [-]
Publicado en
Analytical Methods, v. 6 (June 2014)Derechos de acceso
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
Aparece en las colecciones
- IUPA_Articles [306]