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dc.contributor.authorTaurino, Marco
dc.contributor.authorAbelenda, José A.
dc.contributor.authorRío-Alvarez, Isabel
dc.contributor.authorNavarro, Cristina
dc.contributor.authorVicedo, Begonya
dc.contributor.authorFarmaki, Theodora
dc.contributor.authorJiménez, Pedro
dc.contributor.authorGarcía Agustín, Pilar
dc.contributor.authorLópez-Solanilla, Emilia
dc.contributor.authorPrat, Salomé
dc.contributor.authorRojo, Enrique
dc.contributor.authorSánchez-Serrano, José J.
dc.contributor.authorSanmartín, Maite
dc.date.accessioned2015-07-02T16:41:51Z
dc.date.available2015-07-02T16:41:51Z
dc.date.issued2014
dc.identifier.issn0960-7412
dc.identifier.issn1365-313X
dc.identifier.urihttp://hdl.handle.net/10234/126024
dc.description.abstractThe plant cell wall constitutes an essential protection barrier against pathogen attack. In addition, cell-wall disruption leads to accumulation of jasmonates (JAs), which are key signaling molecules for activation of plant inducible defense responses. However, whether JAs in return modulate the cell-wall composition to reinforce this defensive barrier remains unknown. The enzyme 13–allene oxide synthase (13–AOS) catalyzes the first committed step towards biosynthesis of JAs. In potato (Solanum tuberosum), there are two putative St13–AOS genes, which we show here to be differentially induced upon wounding. We also determine that both genes complement an Arabidopsis aos null mutant, indicating that they encode functional 13–AOS enzymes. Indeed, transgenic potato plants lacking both St13–AOS genes (CoAOS1/2 lines) exhibited a significant reduction of JAs, a concomitant decrease in wound-responsive gene activation, and an increased severity of soft rot disease symptoms caused by Dickeya dadantii. Intriguingly, a hypovirulent D. dadantii pel strain lacking the five major pectate lyases, which causes limited tissue maceration on wild-type plants, regained infectivity in CoAOS1/2 plants. In line with this, we found differences in pectin methyl esterase activity and cell-wall pectin composition between wild-type and CoAOS1/2 plants. Importantly, wild-type plants had pectins with a lower degree of methyl esterification, which are the substrates of the pectate lyases mutated in the pel strain. These results suggest that, during development of potato plants, JAs mediate modification of the pectin matrix to form a defensive barrier that is counteracted by pectinolytic virulence factors from D. dadantii.ca_CA
dc.format.extent11 p.ca_CA
dc.language.isoengca_CA
dc.publisherWileyca_CA
dc.relation.isPartOfThe Plant Journal (2014) 77, 418–429ca_CA
dc.rights© 2013 The AuthorsThe Plant Journal © 2013 John Wiley & Sons Ltdca_CA
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/*
dc.subjectjasmonic acidca_CA
dc.subjectOPDAca_CA
dc.subjectallene oxide synthaseca_CA
dc.subjectcell wallca_CA
dc.subjectpectin methyl-esterificationca_CA
dc.subjectDickeyaca_CA
dc.titleJasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factorsca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1111/tpj.12393
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_CA
dc.relation.publisherVersionhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.12393/epdfca_CA


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