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dc.contributor.authorGauthier, Adrien
dc.contributor.authorTrouvelot, Sophie
dc.contributor.authorKelloniemi, Jani
dc.contributor.authorFrettinger, Patrick
dc.contributor.authorWendehenne, David
dc.contributor.authorDaire, Xavier
dc.contributor.authorJoubert, Jean-Marie
dc.contributor.authorFerrarini, Alberto
dc.contributor.authorDelledonne, Massimo
dc.contributor.authorFlors, Victor
dc.contributor.authorPoinssot, Benoit
dc.date.accessioned2015-06-16T14:08:39Z
dc.date.available2015-06-16T14:08:39Z
dc.date.issued2014
dc.identifier.citationGauthier A, Trouvelot S, Kelloniemi J, Frettinger P, Wendehenne D, Daire X, et al. (2014) The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola. PLoS ONE 9(2): e88145. doi:10.1371/journal.pone.0088145ca_CA
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10234/123736
dc.description.abstractGrapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The b-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this study was to identify the molecular mechanisms of the PS3-induced resistance. For this purpose we studied i) the signaling events and transcriptome reprogramming triggered by PS3 treatment on uninfected grapevine, ii) grapevine immune responses primed by PS3 during P. viticola infection. Our results showed that i) PS3 was unable to elicit reactive oxygen species (ROS) production, cytosolic Ca2+ concentration variations, mitogen-activated protein kinase (MAPK) activation but triggered a long lasting plasma membrane depolarization in grapevine cells, ii) PS3 and Lam shared a common stress-responsive transcriptome profile that partly overlapped the salicylate- (SA) and jasmonate-(JA)-dependent ones. After P. viticola inoculation, PS3 specifically primed the SA- and ROS-dependent defense pathways leading to grapevine induced resistance against this biotroph. Interestingly pharmacological approaches suggested that the plasma membrane depolarization and the downstream ROS production are key events of the PS3-induced resistanceca_CA
dc.format.extent13 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherPublic Library of Scienceca_CA
dc.relation.isPartOfPLoS ONE, February 2014, Volume 9, Issue 2, e88145ca_CA
dc.rights© 2014 Gauthier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ca_CA
dc.rightsAttribution 4.0 Spain*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/*
dc.titleThe Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine’s Induced Resistance against Plasmopara viticolaca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0088145
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_CA
dc.relation.publisherVersionhttp://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0088145&representation=PDFca_CA


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© 2014 Gauthier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Excepto si se señala otra cosa, la licencia del ítem se describe como: © 2014 Gauthier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.