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Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads
dc.contributor.author | Izquierdo Henríquez, Diana F. | |
dc.contributor.author | Barbosa, Oveimar | |
dc.contributor.author | Burguete, M. Isabel | |
dc.contributor.author | Lozano, Pedro | |
dc.contributor.author | Luis, Santiago V. | |
dc.contributor.author | Fernandez Lafuente, Roberto | |
dc.contributor.author | Garcia-Verdugo, Eduardo | |
dc.date.accessioned | 2015-06-15T07:12:59Z | |
dc.date.available | 2015-06-15T07:12:59Z | |
dc.date.issued | 2013-12 | |
dc.identifier.citation | IZQUIERDO, Diana F., et al. Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads. RSC Advances, 2014, 4.12: 6219-6225. | ca_CA |
dc.identifier.uri | http://hdl.handle.net/10234/123484 | |
dc.description.abstract | Lipase B from Candida antarctica (CALB) is able to catalyze C–C bond formation. After immobilization onto a hydrophobic PS-DVB support, the activity increases when compared to that of the soluble or tan – the commercially available Novozyme 435 (being up to 6 fold more active). Our results show that although this activity is not related to the catalytic group, the promiscuous activity of CALB may be tuned via immobilization. In addition, we have show that the secondary structure of both immobilized enzymes is quite different, using FT-ATR-IR spectroscopy. | ca_CA |
dc.format.extent | 6 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Royal Society of Chemistry | ca_CA |
dc.relation.isPartOf | RSC Adv., 2014,4 | ca_CA |
dc.rights | © The Royal Society of Chemistry 2014 | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Lipase B | ca_CA |
dc.subject | Candida antarctica | ca_CA |
dc.subject | C–C bond formation | ca_CA |
dc.title | Tuning lipase B from Candida antarctica C–C bond promiscuous activity by immobilization on poly-styrene-divinylbenzene beads | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/ 10.1039/C3RA47069E | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://pubs.rsc.org/en/Content/ArticleLanding/2014/RA/c3ra47069e#!divAbstract | ca_CA |
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