2024-03-29T11:01:51Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1741882023-10-10T11:53:53Zcom_10234_71345com_10234_158176col_10234_163799
Repositori UJI
author
Olivares-García, Régulo
other
Correa Sanz, Mercè
other
Universitat Jaume I. Departament de Psicologia Bàsica, Clínica i Psicobiologia
2018-04-18T09:22:46Z
2018-04-18T09:22:46Z
2017-09-21
http://hdl.handle.net/10234/174188
Background:
Motivation is characterized by high levels of behavioral activation, vigor,
persistence and goal directed behavior. These functions are impair
ed in pathologies such
as depression or Parkinson disease. Dopamine (DA) in striatum, mainly in nucleus
accumbens (Nacb), plays an important role in the regulation o
f speed or perseverance in
goal
-
motivated behavior. Studies in animals
show that DA depleti
ons
produce a shift in
relative preference between reinforcers that require different levels of behavioral
activation, decreasing time spent on a running wheel (RW), and increasing time spent
eating. Therefore, differences in basal DA levels could underlin
e individual
differences
in selection of active versus passive reinforcers
.
Objectives:
These studies were conducted to explore a potential relationship between
individual differences in voluntary wheel running, striatal (ventral and dorsal) DA
activity, and selection of reinforcers with different levels of behavioral activation and
effort r
equirement. In addition, anxiety, and exploration activity levels were compared
betwe
en different groups of runners.
Methods:
CD1 male mice were used in these experiments.
All animals were assessed in
a
Dark and Light box to evaluate anxiety
-
like behavior
, in an Open Field (OF) to assess
exploration
.
After these tests, and during 4 weeks of testing (2 hours a day/5days a
week), animals were
allowed to run
on
a
running wheel (
RW
)
. Different activity groups
were determined using
first and fou
rth quartiles of
the average running counts. T
he
n
the
th
ree choice T
-
maze task to evaluate spontaneous preferences was used
. After these
tests, brains were collected and expression of DARPP
-
32 phosphorylated at the
threonine (Thr) 34 and
at
Thr
75 residues was assessed
immunohistochemically t
o
provide a marker of DA
receptor related metabotropic activity
.
Results:
In the three
-
choice T
-
maze task
, the high runners spent signif
icantly more time
in the RW. They had a non
-
significant tendency to spent
less ti
me in contact wi
th the
food
.
No significant differences were observed in sniffing time.
There were no
significant differences in anxiety,
or OF exploration.
Finally, pDARPP32
-
Thr34 was
lower among the high runners in t
he 4 areas of striatum studied.
Conclusions:
These res
ults suggest that differences in basal levels of voluntary exercise
are related to preferences for reinforcers that require high levels of activation and effort,
and those could be mediated by an increase in DA D2 receptor stimulation
.
The present
results
have implications for understanding and treating symptoms such as fatigue,
anergia and psychomotor slowing seen in pathologies with a clear motivational
component.
eng
Màster Universitari en Investigació en Cervell i Conducta
Máster universitario en Investigación en Cerebro y Conducta
Master's Degree in Research on the Brain and Behaviour
Individual differences in running wheel performance and preference for active versus passive reinforcers: role of DARPP32
info:eu-repo/semantics/masterThesis
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