2024-03-19T04:33:38Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1211442022-10-14T11:12:13Zcom_10234_8017com_10234_9col_10234_8616
Repositori UJI
author
Ceci, Roberta
author
Duranti, Guglielmo
author
Dimauro, Ivan
author
Quaranta, Federico
author
Pittaluga, Monica
author
Sabatini, Stefania
author
Caserotti, Paolo
author
Parisi, Paolo
author
Parisi, Attilio
author
Caporossi, Daniela
author
Beltran Valls, Maria Reyes
2015-05-18T13:09:55Z
2015-05-18T13:09:55Z
2014
CECI, Roberta, et al. Oxidative stress responses to a graded maximal exercise test in older adults following explosive-type resistance training. Redox biology, 2014, vol. 2, p. 65-72
2213-2317
http://hdl.handle.net/10234/121144
http://dx.doi.org/10.1016/j.redox.2013.12.004
We recently demonstrated that low frequency, moderate intensity, explosive-type resistance training (EMRT) is highly beneficial in elderly subjects towards muscle strength and power, with a systemic adaptive response of anti-oxidant and stress-induced markers. In the present study, we aimed to evaluate the impact of EMRT on oxidative stress biomarkers induced in old people (70–75 years) by a single bout of acute, intense exercise. Sixteen subjects randomly assigned to either a control, not exercising group (n=8) or a trained group performing EMRT protocol for 12-weeks (n=8), were submitted to a graded maximal exercise stress test (GXT) at baseline and after the 12-weeks of EMRT protocol, with blood samples collected before, immediately after, 1 and 24 h post-GXT test. Blood glutathione (GSH, GSSG, GSH/GSSG), plasma malonaldehyde (MDA), protein carbonyls and creatine kinase (CK) levels, as well as PBMCs cellular damage (Comet assay, apoptosis) and stress–protein response (Hsp70 and Hsp27 expression) were evaluated. The use of multiple biomarkers allowed us to confirm that EMRT per se neither affected redox homeostasis nor induced any cellular and oxidative damage. Following the GXT, the EMRT group displayed a higher GSH/GSSG ratio and a less pronounced increase in MDA, protein carbonyls and CK levels compared to control group. Moreover, we found that Hsp70 and Hsp27 proteins were induced after GXT only in EMRT group, while any significant modification within 24 h was detected in untrained group. Apoptosis rates and DNA damage did not show any significant variation in relation to EMRT and/or GXT. In conclusion, the adherence to an EMRT protocol is able to induce a cellular adaptation allowing healthy elderly trained subjects to cope with the oxidative stress induced by an acute exercise more effectively than the aged-matched sedentary subjects.
eng
© 2013 The Authors Attribution-NonCommercial-ShareAlike 4.0 Spain
explosive-type moderate intensity resistance training (EMRT)
graded maximal exercise test (GXT)
oxidative stress
HSPs
apoptosis
elderly
Oxidative stress responses ti a graded maximal exercise test in older adults following explosive-type resistance training
info:eu-repo/semantics/article
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