2024-03-29T11:57:51Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/881092023-06-28T08:03:06Zcom_10234_7013com_10234_9col_10234_8638
00925njm 22002777a 4500
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Zinovjev, Kirill
author
Ruiz-Pernía, José Javier
author
Tuñón, Iñaki
author
2013
We present a combination of the string method and a path collective variable for the exploration of the free energy surface associated to a chemical reaction in condensed environments. The on-the-fly string method is employed to find the minimum free energy paths on a multidimensional free energy surface defined in terms of interatomic distances, which is a convenient selection to study bond forming/breaking processes. Once the paths have been determined, a reaction coordinate is defined as a measure of the advance of the system along these paths. This reaction coordinate can be then used to trace the reaction Potential of Mean Force from which the activation free energy can be obtained. This combination of methodologies has been here applied to the study, by means of Quantum Mechanics/Molecular Mechanics simulations, of the reaction catalyzed by guanidinoacetate methyltransferase. This enzyme catalyzes the methylation of guanidinoacetate by S-adenosyl-l-methionine, a reaction that involves a methyl transfer and a proton transfer and for which different reaction mechanisms have been proposed.
ZINOVJEV, Kirill; RUIZ-PERNÍA, J. Javier; TUÑÓN, Iñaki. Toward an automatic determination of enzymatic reaction mechanisms and their activation free energies. Journal of Chemical Theory and Computation, 2013, 9.8: 3740-3749.
1549-9618
http://hdl.handle.net/10234/88109
http://dx.doi.org/ 10.1021/ct400153r
Enzyme
coenzyme biochemistry
Biochemical Methods
Toward an Automatic Determination of Enzymatic Reaction Mechanisms and Their Activation Free Energies