2024-03-29T08:04:07Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1831112019-12-12T10:21:50Zcom_10234_43662com_10234_9col_10234_43643
00925njm 22002777a 4500
dc
Sturm, Benneth
author
Soldevila, Fernando
author
Tajahuerce, Enrique
author
Gigan, Sylvain
author
Rigneault, Herve
author
de Aguiar, Hilton B.
author
2019-04-25
Compressive Raman is a recent framework that allows for large data compression of microspectroscopy during its measurement. Because of its inherent multiplexing architecture, it has shown imaging speeds considerably higher than conventional Raman microspectroscopy. Nevertheless, the low signal-to-noise ratio of Raman scattering still poses challenges for high-sensitivity bioimaging exploiting compressive Raman: (i) the idle solvent acts as a background noise upon imaging small biological organelles, (ii) current compressive spectrometers are lossy, precluding high-sensitivity imaging. We present inexpensive high-throughput spectrometer layouts for high-sensitivity compressive hyperspectroscopy. We exploit various modalities of compressive Raman allowing for up to 80× reduction of data storage and 2× microspectroscopy speed up at a 230 nm spatial resolution. Such achievements allowed us to chemically image subdiffraction-limited biological specimens (lipid bilayers) in a few seconds, paving the way for applications of a compressive Raman framework in cell biology and nanophotonics.
STURM, Benneth, et al. High-sensitivity high-speed compressive spectrometer for Raman imaging. ACS Photonics, 2019, vol. 6, no 6, p. 1409-1415
2330-4022
http://hdl.handle.net/10234/183111
https://doi.org/10.1021/acsphotonics.8b01643
Raman scattering
microspectroscopy
compressive sensing
label-free
vesicles
membrane
High-Sensitivity High-Speed Compressive Spectrometer for Raman Imaging