2024-03-29T06:48:40Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1705702022-10-28T16:35:29Zcom_10234_7053com_10234_9col_10234_8639
00925njm 22002777a 4500
dc
Izquierdo Arcusa, María Ángeles
author
Wadhavane, Prashant Dinkar
author
Vigara, Laura
author
Burguete, M. Isabel
author
Galindo, Francisco
author
Luis, Santiago V.
author
2017
The fluorescence quenching, by a series of amino acids, of pseudopeptidic compounds acting as probes for cellular acidity has been investigated. It has been found that amino acids containing electron-rich aromatic side chains like Trp or Tyr, as well as Met quench the emission of the probes mainly via a collisional mechanism, with Stern–Volmer constants in the 7–43 M−1 range, while other amino acids such as His, Val or Phe did not cause deactivation of the fluorescence. Only a minor contribution of a static quenching due to the formation of ground-state complexes has been found for Trp and Tyr, with association constants in the 9–24 M−1 range. For these ground-state complexes, a comparison between the macrocyclic probes and an open chain analogue reveals the existence of a moderate macrocyclic effect due to the preorganization of the probes in the more rigid structure.
Photochem. Photobiol. Sci., 2017, 16, 1320
1474-905X
1474-9092
http://hdl.handle.net/10234/170570
http://doi.org/10.1039/c7pp00167c
The interaction of amino acids with macrocyclic pH probes of pseudopeptidic nature