2024-03-29T07:38:09Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1698742024-02-21T10:06:50Zcom_10234_36080com_10234_9com_10234_33596col_10234_36082col_10234_33597
00925njm 22002777a 4500
dc
Fabregat-Safont, David
author
Barneo-Muñoz, Manuela
author
Martinez-Garcia, Fernando
author
Sancho, Juan V
author
Hernandez, Felix
author
Ibáñez, Maria
author
2017-07
New psychoactive substances (NPS) are a new breed of synthetically produced substances designed to mimic the effects of traditional illegal drugs. Synthetic cannabinoids and synthetic cathinones are the two most common groups, which try to mimic the effects of the natural compounds 9Δ-tetrahydrocannabinol and cathinone, respectively. Similarly, synthetic tryptamines are designer compounds which are based on the compounds psilocin, N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine found in some mushrooms. One of the most important tryptamine compounds found in seizures is 5-methoxy-N,N-diisopropyltryptamine, which has been placed as controlled substance in USA and some European countries. The control of this compound has promoted the rising of another tryptamine, the 5-methoxy-N-methyl-N-isopropyltryptamine, which at the time of writing this article has not been banned yet. So, it is undeniable that this new substance should be monitored.
5-methoxy-N-methyl-N-isopropyltryptamine has been reported by the Spanish Early Warning System and detected in our laboratory in two pill samples purchased in a local smart shop. This has promoted the need of stablishing consumption markers for this compound in consumers’ urine. In the present work, the metabolism and pharmacokinetic of 5-methoxy-N-methyl-N-isopropyltryptamine has been studied by an in vivo approach, using adult male mice of the inbred strain C57BLJ/6. The use of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry allowed the identification of four metabolites. After the pharmacokinetic study in serum and urine, the O-demethylated metabolite and the non-metabolised parent compound are proposed as consumption markers in hydrolysed urine. Data reported in this work will help hospitals and forensic laboratories to monitor the consumption and potential intoxication cases related to this tryptamine.
FABREGAT-SAFONT, D., et al. Proposal of 5-methoxy-N-methyl-N-isopropyltryptamine consumption biomarkers through identification of in vivo metabolites from mice. Journal of Chromatography A, 2017.
http://hdl.handle.net/10234/169874
https://doi.org/10.1016/j.chroma.2017.06.010
5-MeO-MiPT
Tryptamines In vivo studies
metabolite identification
high resolution mass spectrometry
new psychoactive substances
Proposal of 5-methoxy-N-methyl-N-isopropyltryptamine consumption biomarkers through identification of in vivo metabolites from mice