2024-03-28T08:47:59Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1447452023-01-30T08:32:15Zcom_10234_33596com_10234_9col_10234_33597
00925njm 22002777a 4500
dc
Pozo, Óscar J.
author
Ibáñez, Maria
author
Sancho, Juan V
author
Lahoz-Beneytez, Julio
author
Farré, Magi
author
Papaseit, Esther
author
De la Torre, Rafael
author
Hernandez, Felix
author
2015-02
In recent years, many new designer drugs have emerged, including the group of cathinone derivatives. One frequently occurring drug is mephedrone; although mephedrone was originally considered as a “legal high” product, it is currently banned in most Western countries. Despite the banning, abuse of the drug and seizures are continuously reported. Although the metabolism of mephedrone has been studied in rats or in vitro using human liver microsomes, to the best of our knowledge, no dedicated study with human volunteers has been performed for studying the in vivo metabolism of mephedrone in humans. Therefore, the aim of this study was to establish the actual human metabolism of mephedrone and to compare it with other models. For this purpose, urine samples of two healthy volunteers, who ingested 200 mg mephedrone orally, were taken before administration and 4 hours after substance intake. The discovery and identification of the phase I and phase II metabolites of mephedrone were based on ultra-high-performance liquid chromatography coupled to hybrid quadrupole time-of-flight mass spectrometry, operating in the so-called MSE mode. Six phase I metabolites and four phase II metabolites were identified, four of them not previously reported in the literature. The structure of four of the detected metabolites was confirmed by synthesis of the suggested compounds. Remarkably, a mephedrone metabolite conjugated with succinic acid has been identified and confirmed by synthesis. According to the reviewed literature, this is the first time that this type of conjugate is reported for human metabolism.
0090-9556
1521-009X
http://hdl.handle.net/10234/144745
http://dx.doi.org/10.1124/dmd.114.061416
Analytical strategies
LC-MS/MS
Waters
4-METHYLMETHCATHINONE
Elucidation
Omeprazole
Fatalities
Pathways
Products
Spectra
Mass Spectrometric Evaluation of Mephedrone In Vivo Human Metabolism: Identification of Phase I and Phase II Metabolites, Including a Novel Succinyl Conjugate