2024-03-29T12:26:25Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1224032023-10-05T12:09:35Zcom_10234_7013com_10234_9col_10234_8638
00925njm 22002777a 4500
dc
Vives-Peris, Vicente
author
Carda-Broch, Samuel
author
Esteve-Romero, Josep
author
2014
This paper describes a micellar liquid chromatographic method used to analyze tamoxifen (TAMO) in pharmaceutical formulations, while focusing in its interesting features. Solid samples were solved in a micellar solution, irradiated at 254 nm, filtered and injected. Extraction steps were avoided and thus expediting the procedure. Tamoxifen was resolved in <5 min, using a mobile phase containing 0.15 M sodium dodecyl sulfate–7% pentanol at pH 3, running at 1.5 mL/min under an isocratic mode at 40°C through a C18 column. Detection was achieved by fluorescence by excitation at 260 nm and emission at 380 nm. The validation was performed following the requirements of the International Conference on Harmonization (ICH) Tripartite Guidelines in terms of: specificity, sensitivity, calibration range (0.2 – 20 mg/L), accuracy (98.8 – 101.7%), precision (<1.5%) and robustness (<6.2%). The method was applied to quantify TAMO in TAMO citrate tablets supplied in Spain, and was found appropriate for the quality control of TAMO formulations
PERIS VICENTE, J.; CARDA BROCH, S.; ESTEVE ROMERO, J. Quantification of Tamoxifen in Pharmaceutical Formulations Using Micellar Liquid Chromatography. Analytical Sciences, v. 30, n. 9 (2014), p. 925-930
http://hdl.handle.net/10234/122403
http://dx.doi.org/10.2116/analsci.30.925
Cancer
Formulation
Micellar
Tamoxifen
Validation
Quantification of Tamoxifen in Pharmaceutical Formulations Using Micellar Liquid Chromatography